IMPROVED METHOD FOR ADENOVIRAL VECTORS CONSTRUCTION

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$129,946.00
Award Year:
2001
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
54517
Agency Tracking Number:
1R43GM062043-01
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
624 N HILLVIEW DR, BOISE, ID, 83713
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
XAVIER DANTHINNE
() -
Business Contact:
(208) 336-1686
XDANTHIN@MICRON.NET
Research Institute:
n/a
Abstract
DESCRIPTION (adapted from applicant's abstract): An innovative system is proposed for the utilization of the adenoviral terminal protein to facilitate the construction of adenoviral vectors used for gene transfer, and in particular gene therapy. So far, adenoviral vectors are best generated using bacterial plasmids, because they eliminate potential viral contaminations. However, plasmid-derived adenoviral DNAs are poorly infectious, and the recovery of the virus sometimes can take several weeks. There is ample evidence in literature, and our preliminary data have confirmed, that the presence of the terminal protein at both ends of the adenoviral DNA increases its infectivity by two or three orders of magnitude. Our goal is to increase the infectivity of adenoviral DNAs prepared from plasmids, by using the terminal protein. We propose 1) a method for purifying the terminal protein and, b) a method to bind the purified protein to the ends of adenoviral DNAs derived from the plasmids. We brought together a strong team and the resources necessary to demonstrate our concept. We expect this development will provide a more expedient and thus less expensive method for the construction of first-generation, second-generation and gutless adenoviral vectors, than currently available. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

* information listed above is at the time of submission.

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