Telomeric DNA Probes for Clinical Diagnostics

Award Information
Agency:
Department of Health and Human Services
Branch:
N/A
Amount:
$750,000.00
Award Year:
1995
Program:
SBIR
Phase:
Phase II
Contract:
N/A
Agency Tracking Number:
19259
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Oncor Inc.
209 Perry Parkway #7, Gaithersburg, MD, 20877
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
N/A
Principal Investigator
 Jeanne Dietz-band
 (301) 963-3500
Business Contact
Phone: () -
Research Institution
N/A
Abstract
MOLECULAR CYTOGENETICS HAS HAD A MAJOR IMPACT ON CHROMOSOME ANALYSIS IN BOTH RESEARCH AND CLINICAL LABORATORIES. TELOMERES, TOGETHER WITH THE CENTROMERE, DEFINE THE PHYSICAL LIMITS OF EACH CHROMOSOME. CHROMOSOME-SPECIFIC CENTROMERE PROBES ARE PRESENTLY AVAILABLE. THESE PROBES, IN CONJUNCTION WITH CHROMOSOME-SPECIFIC TELOMERE PROBES, WOULD ALLOW DETECTION OF ALL MAJOR TRANSLOCATIONS INVOLVING A TELOMERE AS WELL AS SUBMICROSCOPIC TRANSLOCATIONS WHICH ARE STRONGLY INDICATED AS A FREQUENT CAUSE OF MENTAL RETARDATION, INFERTILITY AND MISCARRIAGE, AND SPECIFIC HUMAN CANCERS AND LEUKEMIAS. THE PHASE I EFFORT ADDRESSES THE FEASIBILITY OF DEVELOPING CHROMOSOME-SPECIFIC TELOMERIC PROBES UTILIZING A SOPHISTICATED STRATEGY OF SCREENING HUMAN GENOMIC LIBRARIES TO ISOLATE CLONES FOR BUILDING SETS OF OVERLAPPING COSMIDS (CONTIGS) TO USE AS MOLECULAR PROBES. TELOMERES ON THE SHORT ARMS OF CHROMOSOME 1 (1P),4(4P), AND 17(17P) ARE TARGETED FOR PHASE I. DEVELOPED PROBES WILL BE EVALUATED ON A VARIETY OF CLINICAL SAMPLES. MULTIPLE COLOR ANALYSIS FOR SIMULTANEOUS DETECTION OF MULTIPLE PROBES WILL BE INVESTIGATED. COMPLETION OF PHASE I WILL YIELD CLINICALLY-SIGNIFICANT IN SITU PROBES FOR HUMAN TELOMERES AND ESTABLISH A METHODOLOGY THAT WILL GREATLY ENHANCE ALL LEVELS OF MOLECULAR PROBE DEVELOPMENT.

* information listed above is at the time of submission.

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