Multiplexed-Magnetically Assisted Test Strips (M-MATS)

Award Information
Agency: Department of Defense
Branch: Army
Contract: W911SR-04-P-0085
Agency Tracking Number: A045-022-0135
Amount: $99,969.00
Phase: Phase I
Program: STTR
Awards Year: 2004
Solicitation Year: 2004
Solicitation Topic Code: A04-T022
Solicitation Number: N/A
Small Business Information
4100 N. W. Loop 410, Suite 230, San Antonio, TX, 78229
DUNS: 154088710
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 John Bruno
 Senior Scientist
 (210) 731-0015
Business Contact
 William Henderson
Title: Corporate Financial Officer
Phone: (210) 731-0015
Research Institution
 Texas State University
 Billy C Covington
 601 University Drive
San Marcos, TX, 78666
 (512) 245-2314
 Nonprofit college or university
Some biowarfare (BW) agents infect at extremely low doses and their detection is masked by debris-ridden water. Furthermore, a large variety of BW agents may be disbursed in a water supply, thus complicating detection. Therefore, ultrasensitive, multiplexed detection is required. To address this need, Operational Technologies (OpTech) has recently been awarded an EPA SBIR contract to couple highly specific DNA aptamers to ultrasensitive fluorescent nanoparticles (NPs) and push sensitivity limits. However, OpTech's EPA project will demonstrate individual aptamer-NP assays in separate lanes of a Magnetically-Assisted Test Strip (MATS) using a prototype compact fluorescence reader. MATS is similar to an immunochromatographic test strip, but its flow is not limited by capillary action, because it uses magnetically guided aptamer-magnetic microbeads in microfluidic channels to purify targets from the sample. Under this STTR proposal, OpTech proposes to team with Texas State University and leverage its EPA project to accelerate and enhance the MATS-sensor development for both military and EPA use by developing a larger repertoire of multiplexed aptamer-NP and antibody-NP assays using different colored NPs in each microchannel of the MATS. In Phase II, OpTech and Texas State University will build a lifetime fluorometer attachment to the compact sensor for even greater sensitivity.

* Information listed above is at the time of submission. *

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