CW Agent FRET-Aptamer Spray & Compact Scanner

Award Information
Agency: Department of Defense
Branch: Defense Advanced Research Projects Agency
Contract: W31P4Q-06-C-0047
Agency Tracking Number: 05SB2-0416
Amount: $98,994.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solicitation Year: 2005
Solicitation Topic Code: SB052-025
Solicitation Number: 2005.2
Small Business Information
OPERATIONAL TECHNOLOGIES CORP.
4100 N. W. Loop 410, Suite 230, San Antonio, TX, 78229
DUNS: 154088710
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 John Bruno
 Senior Scientist
 (210) 731-0015
 john.bruno@otcorp.com
Business Contact
 William Henderson
Title: Corporate Financial Officer
Phone: (210) 731-0000
Email: bhenders@otcorp.com
Research Institution
N/A
Abstract
Operational Technologies Corporation (OpTech) will develop and demonstrate a cocktail of several Fluorescence Resonance Energy Transfer (FRET)-DNA aptamer sequences of different analyte specificity in a single spray during Phase I for low-level detection of low-volatility chemical warfare (CW) agent simulants on surfaces and equipment. The fluorophore and quencher will be incorporated into a single DNA chain aptamer structure so that fluorescent light will be emitted ("lights on") or quenched ("lights off") only if the specific CW simulant binds to the FRET-aptamer. In Phase II, OpTech will clone and sequence the CW agent aptamers, and develop a compact surface scanner in conjunction with its subcontractor (Taboada Research Instruments). Upon completion of Phase II, OpTech will deliver the integrated fluorometric scanner and FRET-aptamer spray system to DARPA for further testing and validation.

* information listed above is at the time of submission.

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