FRET-Aptamer Assays & Handheld Reader for Nerve Agent Metabolites

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: W81XWH-06-C-0366
Agency Tracking Number: C061-104-0049
Amount: $69,996.00
Phase: Phase I
Program: SBIR
Awards Year: 2006
Solicitation Year: 2006
Solicitation Topic Code: CBD06-104
Solicitation Number: 2006.1
Small Business Information
OPERATIONAL TECHNOLOGIES CORPORATION
4100 N. W. Loop 410, San Antonio, TX, 78229
DUNS: 154088710
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 John Bruno
 (210) 731-0015
 john.bruno@otcorp.com
Business Contact
 William Henderson
Phone: (210) 731-0000
Email: bhenders@otcorp.com
Research Institution
N/A
Abstract
Operational Technologies Corporation (OpTech) proposes to couple the speed and ultrasensitivity of one-step (“bind and detect”) fluorescence resonance energy transfer (FRET) detection with the high affinity and selectivity of DNA aptamers (surrogate antibodies) to detect the phosphonic acid derivative metabolites of organophosphorous nerve agents in urine, blood, and other body fluids. In Phase I, OpTech will develop assays for the known metabolites of Sarin (GB) and Soman (GD, methyl phosphonic, isopropylmethyl phsophonic, and methylpinococyl phosphonic acids). By the end of Phase I, OpTech will demonstrate the use of these FRET-aptamer assays in freeze-dried form in plastic cuvettes with an off-the-shelf, commercially available, battery-operated, handheld fluorometer. In Phase II, OpTech will refine the existing metabolite assays and add metabolite assays for Tabun (GA), VX, and any other nerve agent metabolites of interest. In addition, in Phase II OpTech will partner with a well-known local optoelectronics engineering firm to produce its own version of the handheld fluorometer, which will meet military specifications. OpTech will then deliver the integrated sensor system to the Army for independent testing and evaluation.

* information listed above is at the time of submission.

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