Handheld FRET-Aptamer Sensor for Bone Markers

Award Information
Agency:
National Aeronautics and Space Administration
Branch
n/a
Amount:
$99,948.00
Award Year:
2008
Program:
SBIR
Phase:
Phase I
Contract:
NNX08CC16P
Agency Tracking Number:
074375
Solicitation Year:
2007
Solicitation Topic Code:
X12.01
Solicitation Number:
n/a
Small Business Information
Operational Technologies Corporation
4100 N.W. Loop 410, San Antonio, TX, 78229-4253
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
154088710
Principal Investigator:
John Bruno
Principal Investigator
(210) 731-0015
john.bruno@otcorp.com
Business Contact:
Judson Miner
Business Official
(210) 731-0015
jay.miner@otcorp.com
Research Institution:
n/a
Abstract
Astronauts lose approximately 1-1.5% of their bone mass per month during space travel due to a lack of physical stress in the microgravity environment. Although, no effective treatments or prophylactic regimens have yet been defined, it is important to monitor the bone loss process in space. As such, the sensor must be compact and facile to operate. Therefore, OpTech proposes to extend its already successful and patent-pending competitive fluorescence resonance energy transfer (FRET)-aptamer assay technology to the detection of bone loss and formation markers such as osteocalcin fragments, hydroxylysine, hydroxyproline, C-terminal and N-terminal telopeptides. In Phase I, OpTech will develop, clone and sequence aptamers to each of these markers. OpTech will also incorporate fluorophore-labeled dUTP into the sequenced aptamers by asymmetric PCR and complex them to their quencher-labeled bone markers for testing in buffer, animal sera, and urine. Finally, in Phase I OpTech will dry and reconstitute the assays that will be tested using a commercially available handheld, battery-operated fluorometer and validated using OpTech's spectrofluorometer. In Phase II, the FRET-aptamer assays will be optimized and packaged in special leak-proof sealed plastic cuvettes and delivered to NASA along with the handheld fluorometer for testing on the ISS or other space missions.

* information listed above is at the time of submission.

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