Handheld Aptamer-Magnetic Bead-Quantum Dot Assays for Crimean-Congo Fever

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-09-C-0029
Agency Tracking Number: A083-181-0021
Amount: $119,942.00
Phase: Phase I
Program: SBIR
Awards Year: 2009
Solicitation Year: 2008
Solicitation Topic Code: A08-181
Solicitation Number: 2008.3
Small Business Information
Operational Technologies Corporation
4100 N. W. Loop 410, Suite 230, San Antonio, TX, 78229
DUNS: 154088710
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 John Bruno
 Senior Scientist
 (210) 731-0000
 john.bruno@otcorp.com
Business Contact
 William Henderson
Title: Chief Financial Officer
Phone: (210) 731-0000
Email: bhenders@otcorp.com
Research Institution
N/A
Abstract
Operational Technologies (OpTech) has already developed rapid (< 15 minute) ultrasensitive one-step DNA aptamer-magnetic bead (MB) plus aptamer-quantum dot (QD) sandwich assays for a foodborne pathogen that is quantified with a commercially available handheld and battery-operated fluorometer. OpTech now proposes to extend this rapid and highly portable sensor technology with lyophilized plastic cuvette assays to detection of Crimean-Congo Hemorrhagic (CCHF) virus in body fluids in the field. To avoid the hazards of working with CCHF in Phase I, OpTech will develop, clone and sequence a family of high affinity DNA aptamers to accessible peptide epitopes from the RFV surface that are described in the literature. These aptamer sequences will be screened and used in patent-pending one-step plastic cuvette-adherent aptamer-MB-aptamer-QD sandwich assays along with a commercially available handheld fluorometer. At the end of Phase I, OpTech will deliver 100 lyophilized prototype CCHF assays to the Army for testing and evaluation. In Phase II, OpTech will optimize the assays with respect to specificity and sensitivity. OpTech will then package the lyophilized assays according DoD requirements while beginning to seek FDA approval and deliver the final product for further testing by the Army.

* information listed above is at the time of submission.

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