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E.coREADi: Automated Microbial Assays for Drinking Water Analysis

Award Information
Agency: Department of Defense
Branch: Defense Health Agency
Contract: W81XWH-21-C-0012
Agency Tracking Number: H2S-0009
Amount: $1,707,985.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: N121-053
Solicitation Number: 12.1
Solicitation Year: 2012
Award Year: 2023
Award Start Date (Proposal Award Date): 2023-09-19
Award End Date (Contract End Date): 2024-09-14
Small Business Information
301 1st Street SW Suite 200
Roanoke, VA 24011-1921
United States
DUNS: 627132913
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Nathaniel Talley
 (434) 972-9951
Business Contact
 Maggie Hudson
Phone: (540) 769-8400
Research Institution

The E.coREADi technology leverages a proprietary enrichment method with high specificity and sensitivity fluorescent enzyme activity assays to detect target organisms. Originally developed as an assay kit to facilitate E. coli testing within wastewater effluents aboard US Navy vessels and ensure EPA compliance prior to discharging treated water, the E.coREADi technology is currently being refined for automated drinking water analysis. Ongoing development has included modifying the microbiological assay to integrate simultaneous detection of coliform bacteria, as well as reagent and protocol optimizations for recovering chlorine-stressed organisms. Luna Labs is currently transitioning the assay to an automated microfluidic system that will simplify operational requirements and facilitate field use. The system includes low-cost and injection-molded microfluidic cartridges (Figure 1) manufactured with shelf-stable reagents, and a ruggedized low-footprint, battery operated, and portable benchtop instrument to automate the system. For the end-user, the test sample is simply added to the cartridge and inserted to the instrument, with on-screen prompts enabling high throughput analysis of up to 16 samples and same-day results to be received electronically.

* Information listed above is at the time of submission. *

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