PEROXIDASE ACTIVATION

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
4947
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Oxford Biomedical Research Inc
Po Box 522, 1600 Hosner Road, Oxford, MI, 48051
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ARTHUR W BULL, JR
PRINCIPAL INVESTIGATOR
(313) 628-5104
Business Contact:
() -
Research Institution:
n/a
Abstract
ASSAYS THAT EMPLOY MAMMALIAN ENZYMES (GENERALLY MIXED- FUNCTION OXIDASES FROM RAT LIVER) TO PROVIDE METABOLIC ACTIVATION OF PREMUTAGENS TO ULTIMATE MUTAGENS HAVE BEEN DEVELOPED AND ARE WIDELY USED TO IDENTIFY MUTAGENIC COMPOUNDS. IT IS NOW CLEAR THAT SOME COMPOUNDS ARE ACTIVATED BY OTHER OXIDIZING ENZYMES, SUCH AS PEROXIDASES, THAT ARE NOT PRESENT IN RAT LIVER S-9 PREPARATIONS. HOWEVER, THE USE OF PEROXIDASES IN ROUTINE MUTAGENICITY SCREENING HAS BEEN LIMITED BY THE COMMERCIAL UNAVAILABILITY OF MAMMALIAN PEROXIDASES. THIS PROJECT WILL DEVELOP AND COMMERCIALIZE A SHORT-TERM MUTAGENICITY ASSAY THAT EMPLOYS AMAMMALIAN PEROXIDASE TO PROVIDE METABOLIC ACTIVATION AND SALMONELLA TYPHIMURIUM TO DETECT MUTAGENS. IN PHASE I, RESEARCHERS WILL DEVELOP CONDITIONS TO MAXIMALLY ACTIVATE A SERIES OF AROMATIC AMINES TO MUTAGENS BY HIGHLY PURIFIED PROSTAGLANDIN H SYNTHASE. THE SAME CONDITIONS WILL BE USED IN PHASE II TO SCREEN A VARIETY OF STRUCTURALLY UNRELATED COMPOUNDS FOR PEROXIDASE-INDUCED MUTAGENICITY. THESE EXPERIMENTS WILL PROVIDE THE BASIS FOR A NEW MUTAGENICITY SCREENING SYSTEM BASED ON AN ALTERNATIVE PATHWAY OF METABOLIC ACTIVATION.

* information listed above is at the time of submission.

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