Prostaglandin Synthase-2--Purification and Immunoassay

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 DK47855-1,
Agency Tracking Number: 25023
Amount: $377,998.00
Phase: Phase II
Program: SBIR
Awards Year: 1996
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Box 522, 1600 Hosner Road, Oxford, MI, 48371
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Hyesook Kim
 (313) 628-5104
Business Contact
Phone: () -
Research Institution
N/A
Abstract
This project will develop methods for large scale production and quantification of humanprostaglandin H synthase-2 (PGHS-2), a recently discovered enzyme that is differentially regulated andhas significantly different properties than the pGHS-1. Data obtained to date in experimental systemssuggest that the PGHS-2 isoform plays a significant role in inflammation, reproduction, and possiblycarcinogenesis. Methods for large scale production of human PGHS-2 cDNA as the source of humanPGHS-2. Availability of large quantities of purified, metabolically active human PGHS-2 will permitaccelerated development of non-steroidal anti-inflammatory drugs (NSAIDs) that are selective forPGHS-2, as well as the mRNA molecules that encode these enzymes, would permit the monitoring ofthe levels of these enzymes in platelets, leukocytes, and other clinical specimens. Specific enzymeimmunoassays for these enzymes will accelerate research on the differential expression of PGHSisoforms in different disease states, and to monitor individuals taking low dose aspirin based on itsreported antithrombotic and/or anticancer activities.

* Information listed above is at the time of submission. *

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