Development of Antibodies for Drug Metabolizing Enzymes

Award Information
Agency:
Department of Health and Human Services
Amount:
$419,964.00
Program:
SBIR
Contract:
N/A
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
1994
Phase:
Phase II
Agency Tracking Number:
22216
Solicitation Topic Code:
N/A
Small Business Information
Oxford Biomedical Research,
P. O. Box 522, 1600 Hosner Roa, Oxford, MI, 48371
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 Kim Hyesook
 (810) 628-5104
Business Contact
Phone: () -
Research Institution
N/A
Abstract
We will develop and market a comprehensive collection of antibodies specific for enzymes that play a significant role in drug metabolism . Availability of specific antibodies will greatly facilitate studies of polymorphisms, species and tissue distribution of drug metabolizing enzymes, and the role of individual enzymes in the metabolism of endogenous and xenobiotic chemicals. Widespread availability of specific antibodies will overcome current limitations. Some of these limitations include restricted access to antibodies, differences in specificity between preparations, and lack of specificity for many antibodies. We have already developed several goat antibodies directed against rabbit cytochromes P450; antibodies specific for prostaglandin H synthase, including anti-peptide antibodies specific for the recently discovered mitogen inducible PGHS-2, may exhibit a different spectrum of xenobiotic activation than PGHS-1. We are currently developing anti-peptide antibodies specific for mammalian lipoxygenases, which are also implicated in xenobiotic metabolism. However, we are well aware of the primary need for additional antibodies specific for P450 isoforms. We will therefore: (a) acquire available form-specific monoclonal and polyclonal antibodies by licensing form academic laboratories where possible, (b) to develop a series of anti-peptide antibodies and recombinant protein standards to the major P450 isoforms, and (c) to extensively characterize the specificity and reactivity of these antibodies in standard assays.

* information listed above is at the time of submission.

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