Isolation and Purification of Prostaglandin E Isomerase

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,984.00
Award Year:
1993
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
22196
Agency Tracking Number:
22196
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1600 Hosner Road, P. O. Box 52, Oxford, MI, 48371
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Krishnamoorthy Sankaran
(313) 628-5104
Business Contact:
() -
Research Institute:
n/a
Abstract
Prostaglandin E isomerase (known as PGE2 synthase also) catalyses the isomerization reaction in which prostaglandin endoperoxide (PGH2) is converted to PGE2. The potent biological activities of PGE2 indicate an important role for prostaglandin E isomerase in the pathogenesis of periodontal disease, progression of the tumor, inflammatory and immune responses. Commercial availability of a purified preparation of mammalian; therefore, prostaglandin E isomerase should facilitate basic and pharmaceutical research. This includes the studies of factors involved in regulation of PGE isomerase gene expression, development of novel therapeutic agents for the treatment of cancer, diabetes, and the pathogeny of certain forms of schizophrenia. In Phase I, we will purify prostaglandin E isomerase from ram seminal vesicles (RSV), determine conditions for enzyme assay and storage, and evaluate the cofactor requirements of the purified protein. In Phase II research, we will employ affinity chromatography and immunoaffinity chromatography to purify the enzyme to homogeneity. The purified enzyme will be used for the production of polyclonal antibodies and amino acid sequencing. Polyclonal antibodies and oligonucleotide probes based on a partial amino acid sequence will be prepared and used as reagents for the identification and isolation of molecular clones of the prostaglandin E isomerase gene and its further characterization.

* information listed above is at the time of submission.

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