ENDOGENOUS ACTIVATION OF PREMUTAGENS BY L5178Y CELLS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
11471
Agency Tracking Number:
11471
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
11526 53rd St N, Clearwater, FL, 33520
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institute:
n/a
Abstract
THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO GENERATE A MAMMALIAN CELL LINE THAT ENDOGENOUSLY EXPRESSES METABOLICALLY ACTIVE PROSTAGLANDIN H (PGH) SYNTHASE, A PEROXIDASE ENZYME KNOWN TO BE INVOLVED IN MUTAGEN ACTIVATION. AVAILABILITY OF SUCH A CELL LINE WOULD OVERCOMELIMITATIONS OF CURRENT MUTAGENESIS TESTING METHODS AND LEAD TO RESULTS MORE APPLICABLE TO HUMAN RISK ASSESSMENT. TRANSFECTION OF THE MOUSE LYMPHOMA CELL LINE L5178Y/TK(+/-),A CELL LINE SUITABLE FOR USE IN MUTAGENESIS TESTS, WITH AN AVAILABLE CDNA CLONE CONTAINING THE PGH SYNTHASE GENE WILL BE USED TO GENERATE A STABLY TRANSFECTED CELL LINE POSSESSING THE CHARACTERISTICS DESIRED. IT IS EXPECTED THAT PHASE I OF THIS PROJECT WILL RESULT IN THE PRODUCTION OF A CELL LINE THAT EXPRESSES STABLE AND FUNCTIONALLY ACTIVE PGH SYNTHASE. LOGICAL DEVELOPMENTS FOR PHASE II WORK INCLUDE: (1) SUBCELLULAR LOCALIZATION OF THE EXPRESSED PGH SYNTHASE IN THE TRANSFECTED LYMPHOMA CELLS, AND (2) SCREENING OF CHEMICALS THAT MAY REQUIRE PEROXIDASE ACTIVATION TO EXHIBIT THEIR MUTAGENICITY.

* information listed above is at the time of submission.

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