ENDOGENOUS ACTIVATION OF PREMUTAGENS BY L5178Y CELLS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 11471
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1989
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Oxford Medilog Inc
11526 53rd St N, Clearwater, FL, 33520
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO GENERATE A MAMMALIAN CELL LINE THAT ENDOGENOUSLY EXPRESSES METABOLICALLY ACTIVE PROSTAGLANDIN H (PGH) SYNTHASE, A PEROXIDASE ENZYME KNOWN TO BE INVOLVED IN MUTAGEN ACTIVATION. AVAILABILITY OF SUCH A CELL LINE WOULD OVERCOMELIMITATIONS OF CURRENT MUTAGENESIS TESTING METHODS AND LEAD TO RESULTS MORE APPLICABLE TO HUMAN RISK ASSESSMENT. TRANSFECTION OF THE MOUSE LYMPHOMA CELL LINE L5178Y/TK(+/-),A CELL LINE SUITABLE FOR USE IN MUTAGENESIS TESTS, WITH AN AVAILABLE CDNA CLONE CONTAINING THE PGH SYNTHASE GENE WILL BE USED TO GENERATE A STABLY TRANSFECTED CELL LINE POSSESSING THE CHARACTERISTICS DESIRED. IT IS EXPECTED THAT PHASE I OF THIS PROJECT WILL RESULT IN THE PRODUCTION OF A CELL LINE THAT EXPRESSES STABLE AND FUNCTIONALLY ACTIVE PGH SYNTHASE. LOGICAL DEVELOPMENTS FOR PHASE II WORK INCLUDE: (1) SUBCELLULAR LOCALIZATION OF THE EXPRESSED PGH SYNTHASE IN THE TRANSFECTED LYMPHOMA CELLS, AND (2) SCREENING OF CHEMICALS THAT MAY REQUIRE PEROXIDASE ACTIVATION TO EXHIBIT THEIR MUTAGENICITY.

* information listed above is at the time of submission.

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