THIS PROPOSAL WAS STIMULATED BY THE NIMH PROGRAM ON TWO-DIMENSIONAL ELECTROPHORESIS.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$32,055.00
Award Year:
1984
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
1416
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Peptide Analysis Laboratory
2013 Lakeview Ave, Middleton, WI, 53562
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
HISASHI CHARLES IIDA
() -
Business Contact:
(608) 836-3131
Research Institution:
n/a
Abstract
THIS PROPOSAL WAS STIMULATED BY THE NIMH PROGRAM ON TWO-DIMENSIONAL ELECTROPHORESIS. A HIGH RESOLUTION GEL SYSTEM WILL BE DEVELOPED FOR THE ANALYSIS OF SMALL PEPTIDES IN COMPLEX MIXTURES. THE FIRST DIMENSION WILL UTILIZE ISOELECTRIC FOCUSING, CONTAINING IMMOBILE AMPHOLITES, GLYOXYLAGAROSE, 6M GUANIDIN HC1 AND A FRACTIONATED TRITON X-100. THE SECOND DIMENSION USES MOLECULAR WEIGHT SEPARATION WITH A LINEAR PORE GRADIENT GEL AND CATIONIC SARFACTANT SODIUM CHOLATE (SC) WHICH FORMS A SMALLER MICELLE THAN SDS. THE CRITICAL MICELLE CONCENTRATION WITH SC IS HIGHER AND EASIER TO REMOVE BY WASHING. DETECTION OF THE PEPTIDES WILL USE AN ULTRA SENSITIVE SILVER STAIN, WHICH IS A MODIFICATION OF MERRIL'S METHOD. THE FIXATION STEP FORMERLY DONE IN A HIGH CONCENTRATION OF ACETIC ACID AND ALCOHOL WILL BE CHANGED TO 1.1% AQUEOUS GLUTARALDEHYDE. THE ABOVE SYSTEM WILL BE DISTRIBUTED AS LABORATORY TRIAL KITS AS SOON AS THEY BECOME AVAILABLE. IT IS ANTICIPATED THAT THEY WILL BE UTILIZED FOR BOTH BIOMEDICAL RESEARCH AND IN CLINICAL DIAGNOSTIC APPLICATIONS AS IN THE ANALYSIS OF HORMONAL PEPTIDES IN HUMAN SPINAL FLUID OR IN BLOOD.

* information listed above is at the time of submission.

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