SECONDARY PHENOLIC PRODUCTION.

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: N/A
Agency Tracking Number: 1635
Amount: $36,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1984
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
7327 Oak Ridge Highway, Knoxville, TN, 37931
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 MILTON J. CONSTANTIN
 VICE PRESIDENT
 () -
Business Contact
 Milton J. Constantin
Title: Vice President
Phone: () -
Research Institution
N/A
Abstract
MANY WOODY PLANT SPECIES CANNOT BE MICROPROPAGATED COMMERCIALLY BECAUSE THEY EXHIBIT SUPERINDUCTION OF SECONDARY PHENOLS (POLYPHENOLS) IN RESPONSE TO EXCISION AND/OR THE HIGH LEVELS OF PLANT GROWTH REGULATORS REQUIRED TO INDUCE ORGANOGENESIS. THESE PHENOLICS MAY DENATURE ENZYMES, INTERFERE WITH ELECTRON TRANSPORT SYSTEMS IN CARBOHYDRATE METABOLISM, OR ALTER THE EXPRESSION OF ENDOGENOUS GROWTH SUBSTANCES. BASIC RESEARCH IN PLANT BIOCHEMISTRY AND PHYSIOLOGY HAS PROVIDED MANY TECHNIQUES BY WHICH THE ACCUMULATION OF SECONDARY PLANT PHENOLICS CAN BE REGULATED. WE PROPOSE TO APPLY THOSE TECHNIQUES TO THE PRACTICAL PROBLEM OF MICROPROPAGATING RECALCITRANT WOODY SPECIES. PHASE I WILL INVOLVE LARGE-SCALE SCREENING EXPERIMENTS WITH SUSCEPTIBLE SPECIES INCLUDING PEARS, DOGWOODS, OAKS, WALNUTS AND MAPLES. THE CULTURE MEDIA WILL BE MODIFIED WITH ADDITIVES KNOWN TO: 1) BLOCK THE FORMATION OF O-QUINONES; 2) CONTROL THE LEVEL OF PHENOLIC FORMATION BY PARTIAL INHIBITION OF PHENYLALANINE AMMONIA-LYASE ACTIVITY; 3) CHELATE PHENOLICS IN THE CULTURE MEDIUM OR WITHIN THE PLANT; AND 4) SELECTIVELY INHIBIT TANNIN BIOSYNTHESIS.

* Information listed above is at the time of submission. *

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