Glutamate Biosensors for Ischemia or Single-Cell Studies

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$98,060.00
Award Year:
2004
Program:
SBIR
Phase:
Phase I
Contract:
1R43NS047955-01A1
Award Id:
71801
Agency Tracking Number:
NS047955
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
PINNACLE TECHNOLOGY, INC., 619 E 8TH ST, STE D, LAWRENCE, KS, 66044
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DAVIDJOHNSON
(785) 832-8866
DAVEJ@PINNACLET.COM
Business Contact:
DONNAJOHNSON
(785) 832-8866
DONNAJ@PINNACLET.COM
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): Pinnacle Technology and Dr. George Wilson of the University of Kansas will design a sensor suite capable of measuring physiologically relevant levels of glutamate, in vivo, in untethered small animals, with high spatial resolution and under ischemic conditions. Research has shown that the major cause of cell death following an ischemic stroke is not hypoxia, but radically elevated levels of glutamate (glutamate excitotoxicity). The ischemic event has been well characterized in terms of blood flow and tissue oxygenation, but other than microdialysis, there is no tool currently available to directly measure glutamate excitotoxicity. Microdialysis is of limited use due to its poor temporal resolution. Electrochemical sensors work well for electroactive species, but not for non-electroactive species. Behavioral scientists also seek to make connections between the brain and behavior. For these studies high spatial resolution is needed. In Phase I, needle-type biosensors (180 mu/m diameter) will be developed to detect large concentrations of glutamate during ischemic conditions. High spatial resolution biosensors (76 mu/m diameter), will also be developed for normal oxygen conditions ( >40 torr). In Phase II, the team will develop a 76 mu/m ischemic glutamate sensor and 10 um glutamate sensors for single-cell measurements and associated electronics.

* information listed above is at the time of submission.

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