A TOXIN-DIRECTED APPROACH TO SELECT LETTUCE RESISTANT TO CORKY ROOT ROT

Award Information
Agency:
Department of Agriculture
Branch
n/a
Amount:
$210,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
4635
Agency Tracking Number:
4635
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Plant Genetics Inc. (Currently Plant Genetics Inc)
1930 Fifth St, Davis, CA, 95616
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
JOHN KAO
() -
Research Institution:
n/a
Abstract
THE HOST-SPECIFIC TOXIN PRODUCED BY THE CORKY ROOT ROT CAUSAL BACTERIUM PROVIDES US WITH A TOOL TO SELECT THE DISEASE-RESISTANT LETTUCE FROM A MASS POPULATION OF GERMPLASMS. THE MAJOR OBJECTIVES OF THIS PROPOSED RESEARCH ARE: 1) PURIFY THE HOST-SPECIFIC TOXIN. THE TOXIN WILL BE EXTRACTED FROM THE CRUDE CULTURE FILTRATE IN WHICH THE BACTERIUM HAS BEEN GROWN AND REFINED TO ITS MAXIMAL PURITY BY VARIOUS CHROMATOGRAPHICAL SYSTEMS. 2) DEVELOP AN IN VITRO SELECTION SYSTEM THAT WILL IDENTIFY BOTH SPONTANE OUSLY-OCCURING AND CHEMICALLY-INDUCED MUTANTS THAT ARE RE SISTANT TO THE TOXIN. THE SOURCES OF THE TOXIN-RESISTANT MUTANTS WILL INCLUDE BOTANICAL SEEDS OF LETTUCE, SOMACLONAL VARIANTS DERIVED FROM TISSUE CULTURE AND MUTANTS DERIVED MUTAGENIZED CALLUS TISSUE CULTURE. LETTUCE PLANTS DERIVED THE MUTANT SEEDS OR CALLUS TISSUES WILL BE TESTED FOR THEIR RESISTANCE TO INFECTION BY THE BACTERIUM.

* information listed above is at the time of submission.

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