CLONING OF HYBRID CELERY USING BIOREACTOR TECHNOLOGY

Award Information
Agency:
National Science Foundation
Branch
n/a
Amount:
$47,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
7565
Agency Tracking Number:
7565
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1930 Fifth Street, Davis, CA, 95616
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DAVID A STUART PHD
() -
Business Contact:
() -
Research Institute:
n/a
Abstract
THIS PROPOSAL DESCRIBES RESEARCH ON BIOREACTOR SCALE UP OF PLANT CELL CULTURE DIRECTED TOWARD PLANT PROPAGATION. IN PHASE I RESEARCH, WE PROPOSE TO CLONE A BREEDER'S CROSS HYBRID OF CELERY IN A 1 TO 4 LITER BIOREACTOR. USING THIS SYSTEM, WE PROPOSE TO IDENTIFY THE IMPORTANCE OF AGITATION, AERATION, AND TIME IN LIQUID CULTURE ON THE YIELD AND PERFORMANCE OF DEVELOPING CELERY SOMATIC EMBRYOS. WE FURTHER PROPOSE TO DETERMINE THE LEVEL OF SINGULATION AND SYNCHRONIZATION OF EMBRYOS RESULTING FROM REGENERATION IN THE BIOREACTOR. A METHOD FOR SEPARATING EMBRYOS FROM DEBRIS AND IMMATURE EMBRYOS WILL BE DETERMINED. FINALLY, SEVERAL HUNDRED SOMATIC EMBRYOS WILL BE REGENERATED AND TRANSPLANTED TO THE FIELD. DURING PHASE II RESEARCH, WE PROPOSE TO COMPLETE THE FIELD EVALUATION OF PLANT VARIATION AS WELL AS CONTINUE BIOREACTOR STUDIES. LETTUCE SOMATIC ORGANOGENESIS WILL ALSO BE STUDIED IN A BIOREACTOR BY TESTING METHODS DEVELOPED FOR CELERY DURING LETTUCE BUD REGENERATION.

* information listed above is at the time of submission.

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