Rapid Liquid Crystal Detection of Ras

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43CA117215-01
Agency Tracking Number: CA117215
Amount: $200,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solicitation Year: 2005
Solicitation Topic Code: N/A
Solicitation Number: PHS2005-2
Small Business Information
PLATYPUS TECHNOLOGIES, LLC
Platypus Technologies, Llc, 5520 Nobel Drive, Suite 100, Madison, WI, 53711
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 BARBARA ISRAEL
 (608) 441-2776
 bisrael@platypustech.com
Business Contact
 SUSAN PSCHORR
Phone: (608) 237-1272
Email: SPSCHORR@PLATYPUSTECH.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): We propose to pursue the development of a novel, rapid (<30 min) exquisitely sensitive (femtogram), label-free platform technology using liquid crystals for the quantification of Ras, a key signaling protein. In this proposal, we will develop a method for the rapid capture of Ras from solution onto micro or nano-sized magnetic particles and then detect the captured Ras on nanostructured surfaces using liquid crystals. The approach involves direct detection of Ras, does not require secondary antibodies, and will permit rapid detection of low concentrations of proteins in small sample volumes, such as tumor cell lysates. In Phase 2, we will expand the technology to detection of other signaling proteins as well as simultaneous determination of their state of activation. The elements of this technology are readily adaptable to high throughput systems suitable for the identification and development of therapeutic compounds through an understanding of their action on cell signaling pathways.

* information listed above is at the time of submission.

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