GLUCOSE INDICATOR FOR IN SITU BIOREACTOR MONITORING

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$99,830.00
Award Year:
1997
Program:
SBIR
Phase:
Phase I
Contract:
HL59124-01
Award Id:
39364
Agency Tracking Number:
39364
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
220 RESERVOIR ST, STE 28 B, Needham Heights, MA, 02194
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
KANE, JAMES A
() -
Business Contact:
(781) 449-2284
Research Institute:
n/a
Abstract
The goal of this Small Business Innovation Research project is to demonstrate a fluorescent glucose-indicator chemistry with high thermal stability for construction of an in situ optical glucose sensor to monitor the culture medium of continuous process bioreactors. The specific binding properties. The new polymer will be evaluated for use in a competitive binding glucose assay using fluorescence resonance energy transfer quenching to detect glucose levels in the culture medium. The evaluation will include characterization of the glucose binding polymer for molecular weight and composition and spectrofluorimetric titration of the proposed indicator chemistry with glucose and known culture medium analytes and metabolites. Spectral data will be examined for glucose sensitivity and selectivity over significant reactor concentration ranges. An in situ glucose probe would improve the ability to control the physiological state of glucose substrate reactors while reducing the cost and complexity. The probe would also provide the type of real time glucose concentrations necessary for substrate limiting culture operation. Improvements in bioreactor performance will aid the ongoing efforts to develop new drugs and therapeutic proteins using biotechnology.

* information listed above is at the time of submission.

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