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An In-Line Test for the Colorimetric Determination of Somatic Cell Count

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: 2007-33610-18447
Agency Tracking Number: 2006-00118
Amount: $346,000.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2007
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
337 Tom Brown Road
Moorestown, NJ 08057
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Wai Law
 (856) 231-9922
Business Contact
 Wai Law
Title: President
Phone: (856) 231-9922
Research Institution

In Phase I, PortaScience successfully built an automated prototype unit for determining
the somatic cell count of dairy milk samples using a liquid colorimetric assay. The
prototype unit can perform the following functions: sip milk samples, add buffer/dye
solutions, mix all solutions and generate a voltage reading at a fixed timed interval
yielding quantitative values for the somatic cell count. The agreement with a FOSS
reference instrument was r = 0.93 and the precision of the system was less than 15% CV at
three SCC levels.
In continuing the project, we plan to develop and validate a self-contained In-line
Modular unit. The objectives of Phase II are as follows:
Specific Aims - Chemistry:
I. Scale-up the dye-substrate from a 10 gram to a 50 gram batch size in order to
minimize reagent cost.
II. Develop a cleaning solution for the flow cell.
III. Evaluate several anti-oxidants in the dye-substrate solution to improve stability
beyond the 12 month shelf-life.
IV. Evaluate a new water-soluble dye-substrate.
Specific Aims - Engineering:
I. Optics: Change from fiber optics to an integrated surface mount to obtain optimal
II. Flow Mechanics: Optimize the pump, valves, flow cell, and mixing procedures.
III. Electronics: Integrate the electronics and software into a package suitable for a field
PortaScience has filed a provisional patent protecting the technology and is now in
discussion with several dairy equipment manufacturers. We believe potential annual
market is forty million dollars.

* Information listed above is at the time of submission. *

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