Proteases and tags for protein purification and analysis.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
2006
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM076786-01
Award Id:
79876
Agency Tracking Number:
GM076786
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
POTOMAC AFFINITY PROTEINS, LLC, 11305 DUNLEITH PL, NORTH POTOMAC, MD, 20878
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
BIAO RUAN
(301) 610-9687
ruan@umbi.umd.edu
Business Contact:
(301) 610-9687
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): The overall goal to this project is to create an integrated system for the expression, detection and rapid purification of proteins from cloned genes. The foundation for this system is a highly engineered subtilisin which hydrolyzes substrates in response to a chemical trigger. The subtilisin prodomain (protag) will be developed both for detection of native target protein and as the ligand for affinity purification. The engineered processing subtilisin (psub) will be used both as the binding molecule for affinity purification of protagged fusion proteins and as the processing protease for protag removal. The long range objective is to develop a sophisticated set of tools to facilitate proteomic analysis in the way restriction endonucleases have been used to facilitate genomic analysis.

* information listed above is at the time of submission.

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