Integrated Twin Epi-Flourescence System

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 1R43NS039204-01A2
Amount: $140,051.00
Phase: Phase I
Program: SBIR
Awards Year: 2001
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
3230 DEMING WAY, STE 175, MIDDLETON, WI, 53562
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
Phone: (608) 662-0022
Research Institution
DESCRIPTION (provided by applicant): Cellular studies of learning and memory, and synaptic transmission are significantly hampered by standard optical configurations that were developed decades ago. This is especially true when considering present day, fluorescence microscope technology where a single optical input pathway provides wide-field illumination. For example, the integration of ion imaging with UV photolysis is compromised since focal UV excitation must be forced into the optical pathway that was designed only for epi-fluorescence illumination. The goal of this project is to test the feasibility of developing a twin-illumination pathway for fluorescencemicroscopes to allow the versatility necessary for the experiments of today and tomorrow. We will: 1) Design and build a twin-illumination pathway that will integrate with present day upright microscopes. 2) Test the optical properties of each illumination pathway for flatness of field, full-field illumination and light transmission properties. 3) Determine the reliability of motor control for positioning apertures and a UV transmitting optical fiber coupling mechanism in one of the illumination pathways. 4) Test and evaluate the feasibility of using "pass through optics to monitor the location of laser illumination beams for photolysis. 5) Test the twin-illumination system in photolysis experiments and in studies to monitor fast calcium transients. PROPOSED COMMERCIAL APPLICATION: This twin-IIumination system has many potential applications in science: simultaneous imaging together with focal ablation or photolysis, and by including apertures in one arm of the excitation pathway, it will be possible to use one pathway for restricted field excitation for measurement of fast changes in calcium in neuronal processes.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government