USING MULTIPLEXED LUMINESCENCE TO EXTEND BIOASSAY CAPABILITIES

Award Information
Agency:
National Science Foundation
Branch
n/a
Amount:
$299,328.00
Award Year:
1997
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
21633
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Promega Corp.
2800 Woods Hollow Rd, Madison, WI, 53711
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Keith V Wood
(608) 274-4330
Business Contact:
() -
Research Institution:
n/a
Abstract
CURRENT LUMINESCENCE BIOASSAYS RELY SOLELY UPON MESUREMENT OF LUMINESCENCE INTENSITY TO GENERATE INFORMATION. MULTIPLE COLORS FROM BEETLE LUCIFERASE HAS CREATED THE OPPORTUNITY TO INCREASE ASSAY INFORMATION CONTENT BY USING MULTIPLEXING TECHNIQUES TO MEASURE LIGHT INTENSITIES AT DIFFERENT WAVELENGTHS. THIS PROVIDES GREATER ASSAY RELIABILITY, PRECISION, AND ABILITY TO MEASURE CONCURRENT MOLECULAR EVENTS. THE EXCELLENT PERFORMANCE OF BEETLE LUCIFERASE ENZYMES IN BIOASSAY METHODS YIELDS THE HIGHEST QUANUM EFFICIENCY OF ANY LUMINESCENCE METHODOLOGY, AND DEMONSTRATES SUPERIOR PRECISION, LINEARITY, AND RAPIDITY. TO FURTHER ASSESS THE FEASIBILITY OF USING MULTIPLEXING TO INCRASE THE COMMERCIAL POTENTIAL OF BIOLUMINESCENCE ASSAYS, RESEARCHERS ARE IDENTIFYING NOVEL SUBSTITUTIONS THAT REGULATE COLOR BY EXAMINING THE RELATIONSHIP BETWEEN AMINO ACID SUBSTITUTIONS IN THE PRIMARY STRUCTURES OF BETLE LUCIFERASES TO THE ENZYMES' SPECTRAL PROPERTISE.

* information listed above is at the time of submission.

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