Developing Technologies for Studying Protein Prenylation

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$98,782.00
Award Year:
1995
Program:
SBIR
Phase:
Phase I
Contract:
1 R43 GM52807-01,
Agency Tracking Number:
29458
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Promega Corp.
2800 Woods Hollow Road, Madison, WI, 53711
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Erik Schaefer
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
Prenylation (the modification of proteins with the cholesterol biosynthesis intermediates C(15) fargeranylgeranyl) is a newly discovered postranslational modification that targets certain proteins tointerior of the cell, thereby regulating their biological activity. The best studied example illustrmodification is the farnesylation of p21ras, the protein encoded for by the ras oncogene which is imnumber of human cancers. Farnesylation of p21ras is required for the plasma membrane localization anactivity of this protein. Our primary goal is to generate antisera that specifically recognize the fmoiety. Antibodies to prenylated cysteine (which are not currently available) will allow rapid detecproteins using standard procedures (e.g., SDS-PAGE, Western blotting, immunoprecipitation, ELISA). Tinclude (i) synthesize prenylated amino acid and peptide derivatives, (ii) couple prenylated derivatand (iii) immunize chickens and rabbits and screen sera for antibodies that recognize farnesylated aproteins.

* information listed above is at the time of submission.

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