HIGH-RESOLUTION, TWO-DIMENSIONAL ELECTROPHORESIS CONSTITUTESTHE MOST POWERFUL TOOL AVAILABLE FOR THE COMPREHENSIVE ANALYSIS OF COMPLEX PROTEIN MIXTURES.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
4949
Agency Tracking Number:
4949
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
12301 Parklawn Drive, Rockville, MD, 20852
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
NORMAN LEIGH ANDERSON
PRINCIPAL INVESTIGATOR
(301) 881-2600
Business Contact:
() -
Research Institution:
n/a
Abstract
HIGH-RESOLUTION, TWO-DIMENSIONAL ELECTROPHORESIS CONSTITUTESTHE MOST POWERFUL TOOL AVAILABLE FOR THE COMPREHENSIVE ANALYSIS OF COMPLEX PROTEIN MIXTURES. THE TECHNIQUE HAS PROVEN EXTREMELY USEFUL IN A VARIETY OF AREAS, INCLUDING COMPARISON OF CELL TYPES, COMPARISON OF SPECIES, ANALYSIS OFDIFFERENTIATION, AND THE SEARCH FOR GENETIC VARIANTS. IN MANY APPLICATIONS, IT IS NECESSARY TO EXAMINE MODERATELY LARGE NUMBERS OF SAMPLES UNDER CONDITIONS THAT PROVIDE VERY HIGH ANALYTICAL REPRODUCIBILITY. SUCH REPRODUCIBILITY COULDBEST BE ACHIEVED USING AUTOMATED SYSTEMS FOR THE PRODUCTION OF BOTH FIRST- AND SECOND-DIMENSION GELS. THIS PROJECT DEALS WITH THE DEVELOPMENT OF A MICROCOMPUTER-CONTROLLED PUMPING DEVICE AND ASSOCIATED GEL-FORMING HARDWARE FOR THE PURPOSE OF PRODUCING LARGE NUMBERS OF VERY REPRODUCIBLE SDS ACRYLAMIDE GRADIENT SLAB GELS. THIS DEVICE CAN MAKE A MAJORCONTRIBUTION TOWARDS STANDARDIZING THE 2-D ELECTROPHORETIC TECHNIQUE, AND THUS FACILITATE THE CONSTRUCTION AND USE OF COMPREHENSIVE PROTEIN INDEXES.

* information listed above is at the time of submission.

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