SERUM-FREE MEDIUM FOR LAK AND NATURAL KILLER CELLS AND MONOCYTES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
7934
Agency Tracking Number:
7934
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
7920 Queenair Dr, Gaitherburg, MD, 20879
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
THE LONG RANGE GOAL OF THE PROPOSED RESEARCH IS TO DEVELOP SERUM-FREE MEDIA FORMULATIONS FOR THE CULTURE OF HUMAN LEUKOCYTES IN VITRO. THE OBJECTIVE OF PHASE I STUDIES IS TODEVELOP SERUM-FREE MEDIUM THAT WOULD ALLOW MAXIMUM GROWTH AND CYTOTOXIC ACTIVITY OF NON-MITOGEN ACTIVATED HUMAN LYMPHOKINE ACTIVATED KILLER CELLS, NATURAL KILLER CELLS, MONOCYTES AND MITOGEN ACTIVATED CYTOTOXIC T CELLS. BASED ON PHASE I RESULTS, PHASE II STUDIES WILL CONCENTRATE ON PRODUCING SERUM-FREE MEDIUM FOR THE BATCH PRODUCTION OF CANCER PATIENT-DERIVED LEUKOCYTES FOR MEDICAL RESEARCH. THIS MEDIUM WOULD ALLEVIATE MANY OF THE PROBLEMS ASSOCIATED WITH CELL PROPAGATION IN THE PRESENCE OF ANIMAL SERA OR POOLED HUMAN SERA. THIS PHASE I PROPOSAL OUTLINES THE DEVELOPMENT OF A HIGH QUALITY SERUM-FREE MEDIUM UTILIZING BIOLOGICAL COMPONENTS DERIVED SOLELY FROM HUMAN SOURCES. THE COMPOSITION AND OPTIMAL CONCENTRATIONS OF EACH COMPONENT OF THE MEDIUM WILL BE SELECTED WITH PARTICULAR CONCERN FOR THE GROWTH AND MAINTENANCE OF HUMAN CELLS. BY CONSTROLLING THE EXTERNAL SIGNALS THE ROLE VARIOUS BIOLOGICAL RESPONSE MODIFIERS PLAY ON CYTOTOXIC ACTIVITY CAN BE CLEARLY EVALUATED.

* information listed above is at the time of submission.

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