Generating Cloned Attenuated Dengue 1 Viral cDNA Sequences

Award Information
Agency: Department of Defense
Branch: Navy
Contract: N/A
Agency Tracking Number: 25684
Amount: $750,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1995
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
7581 Lindbergh Drive, Gaithersburg, MD, 20879
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Sandra K. Dusing
 (301) 840-9331
Business Contact
Phone: () -
Research Institution
Wild type (wt) dengue 1 viral RNA will be used as substrate to generate a complementary DNA (cDNA) library of clones that contain identified and sequenced DNA segments spanning the entire dengue 1 genome. Point mutations designed to generate amino acid alterations will be introduced at the nucleotide level in the cloned dengue 1 DNA sequences by polymerase chain reaction amplification. These mutations will be based upon sequence alterations that have previously been identified by Naval medical researachers in non-virulent or attenuated dengue 1 strains as potentially useful for development of an attenuated dengue 1 vaccine. In Phase II, mutagenized cDNA clones will be combined with appropriate cloned parental wt dengue 1 sequences to produce full-length chimeric dengue 1 cDNAs. These full-length viral cDNAs will subsequently be transcribed into infectious dengue 1 viral RNAs containing mutations of interest for development of a live attenuated dengue 1 viral vaccine and for analysis of dengue 1 pathogenicity and immunogenicity.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government