Generating Cloned Attenuated Dengue 1 Viral cDNA Sequences

Award Information
Agency:
Department of Defense
Branch
Navy
Amount:
$750,000.00
Award Year:
1995
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
25684
Agency Tracking Number:
25684
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
7581 Lindbergh Drive, Gaithersburg, MD, 20879
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Sandra K. Dusing
(301) 840-9331
Business Contact:
() -
Research Institution:
n/a
Abstract
Wild type (wt) dengue 1 viral RNA will be used as substrate to generate a complementary DNA (cDNA) library of clones that contain identified and sequenced DNA segments spanning the entire dengue 1 genome. Point mutations designed to generate amino acid alterations will be introduced at the nucleotide level in the cloned dengue 1 DNA sequences by polymerase chain reaction amplification. These mutations will be based upon sequence alterations that have previously been identified by Naval medical researachers in non-virulent or attenuated dengue 1 strains as potentially useful for development of an attenuated dengue 1 vaccine. In Phase II, mutagenized cDNA clones will be combined with appropriate cloned parental wt dengue 1 sequences to produce full-length chimeric dengue 1 cDNAs. These full-length viral cDNAs will subsequently be transcribed into infectious dengue 1 viral RNAs containing mutations of interest for development of a live attenuated dengue 1 viral vaccine and for analysis of dengue 1 pathogenicity and immunogenicity.

* information listed above is at the time of submission.

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