Generating Cloned Attenuated Dengue 1 Viral cDNA Sequences

Award Information
Agency: Department of Defense
Branch: Navy
Contract: N/A
Agency Tracking Number: 25684
Amount: $750,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1995
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Quality Biological, Inc.
7581 Lindbergh Drive, Gaithersburg, MD, 20879
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Sandra K. Dusing
 (301) 840-9331
Business Contact
Phone: () -
Research Institution
N/A
Abstract
Wild type (wt) dengue 1 viral RNA will be used as substrate to generate a complementary DNA (cDNA) library of clones that contain identified and sequenced DNA segments spanning the entire dengue 1 genome. Point mutations designed to generate amino acid alterations will be introduced at the nucleotide level in the cloned dengue 1 DNA sequences by polymerase chain reaction amplification. These mutations will be based upon sequence alterations that have previously been identified by Naval medical researachers in non-virulent or attenuated dengue 1 strains as potentially useful for development of an attenuated dengue 1 vaccine. In Phase II, mutagenized cDNA clones will be combined with appropriate cloned parental wt dengue 1 sequences to produce full-length chimeric dengue 1 cDNAs. These full-length viral cDNAs will subsequently be transcribed into infectious dengue 1 viral RNAs containing mutations of interest for development of a live attenuated dengue 1 viral vaccine and for analysis of dengue 1 pathogenicity and immunogenicity.

* information listed above is at the time of submission.

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