Reagents for Vector Production for Hematopoietic Gene TX

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$81,000.00
Award Year:
1994
Program:
SBIR
Phase:
Phase I
Contract:
1 R43 CA63979-1,
Award Id:
24873
Agency Tracking Number:
24873
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
7581 Lindbergh Drive, Gaithersburg, MD, 20879
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Ronald Brown
(301) 840-9331
Business Contact:
() -
Research Institute:
n/a
Abstract
The goal of this research is to develop critical reagents that will support the production of highvector titers for transfusion medicine, ie. hematopoietic gene therapy for treatment of blood disordersas well as other applications. Attempts at concentrating these vectors has generally resulted in asignificant loss of infectious activity. One possibility is that the virus titer may be dependent upon thecomposition of the medium in which the viral producing cells (VPC) are grown; therefore, we will studydifferent media compositions in an effort to improve the vector titer produced by the VPC. A secondissue for the medium design is the safety and infusibility of the medium into human patients. The abilityto produce the vector supernatant in reagent medium would minimize the risk of immunizing the patientagainst foreign serum proteins in the media that might produce toxicities that could limit the usefulnessof the therapy. This would allow the direct injection of high titer virus and reduce the treatment timerequired for injected autologous VPC to produce the vectors. As a model system the PA3l7/LNL6packaging cell line that contains the LNL6 vector will be employed. The LNL6 vector carries the bacterialgene for neomycin resistance. Vector titer will be assessed using G418 resistant colony forming unitsby the G418 selection of infected NIH-3T3 fibroblasts. The regent medium will be developed with theeventual goal of producing a medium that is destined for direct infusibility into human patients.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government