Microfluidic Yeast-based Impedance Sensor for Food Monitoring

Award Information
Agency:
Department of Defense
Branch
Office of the Secretary of Defense
Amount:
$99,916.00
Award Year:
2010
Program:
SBIR
Phase:
Phase I
Contract:
W81XWH-10-C-0191
Award Id:
96893
Agency Tracking Number:
O093-H24-3053
Solicitation Year:
n/a
Solicitation Topic Code:
OSD 09-H24
Solicitation Number:
n/a
Small Business Information
215 Wynn Dr., 5th Floor, Huntsville, AL, 35805
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
185169620
Principal Investigator:
Ketan Bhatt
Research Engineer
(256) 327-0666
tsb@cfdrc.com
Business Contact:
Deborah Phipps
Senior Contracts Specialist
(256) 726-4884
dap@cfdrc.com
Research Institution:
n/a
Abstract
Current methods for detecting toxicity in food are labor intensive, time-consuming and require access to sophisticated laboratory equipment. To overcome these limitations we propose to develop a novel, microfluidics yeast-based sensor system for detection of Toxic Industrial Chemicals (TICs) in food. Robust yeast cell wires and membrane patches will be assembled on-demand and in-situ from suspensions of genetically modified yeast cells and functionalized colloidal micro- and nano-particles using dielectrophoretic forces. Presence of TICs will be detected by monitoring the impedance changes of the cell wires and patches. During Phase I, proof-of-concept will be demonstrated for detection of TICs in flour, ground beef and milk samples spiked with arsenic, methamidophos and cyanide. A conceptual design for the Phase II end-product will be developed. During Phase II, the sensor technology will be demonstrated for multiple TICs of interest. The device functionality will be demonstrated for reliable operation through the range of environmental conditions (temperature, relative humidity, etc.) We have assembled an interdisciplinary team of engineers and scientists from CFDRC, UAHuntsville and NC State University with experience in microfluidics, colloid and nanoscale engineering, fungal and plant molecular biology and functional genomics, and sensor fabrication to successfully develop the proposed device.

* information listed above is at the time of submission.

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