IMMOBILIZED ARTIFICIAL MEMBRANE (IAM) CHROMATOGRAPHY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$499,004.00
Award Year:
1993
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
19243
Agency Tracking Number:
19243
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
8210 North Austin Avenue, Morton Grove, IL, 60053
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Louis J Glunz
(708) 967-6000
Business Contact:
() -
Research Institution:
n/a
Abstract
MEMBRANE PROTEIN STUDIES ARE SEVERELY LIMITED BY LACK OF EFFICIENT METHODS FOR THEIR PURIFICATION. IAM SURFACES HAVE RECENTLY BEEN DISCOVERED AND ARE EXTREMELY EFFECTIVE AT PURIFYING FUNCTIONAL MEMBRANES PROTEINS. SINCE IAM SURFACES ARE PREPARED BY COVALENTLY BONDING MEMBRANE-FORMING-LIPIDS TO SILICA, THE IAM CHROMATOGRAPHIC SURFACEEMULATES THE CELL MEMBRANE'S LIPID ENVIRONMENT. CONSEQUENTLY, IAM SURFACES ARE ALSO USEFUL AT PREDICTING SOLUTE AND/OR DRUG TRANSPORT ACROSS BIOLOGICAL BARRIERS. THE SPECIFIC AIMS OF PHASE I STUDIES ARE: (1) TO PREPARE A WIDE PORE (1000 ANGSTROMS) VERSIONS OF THE COMMERCIAL IAM.PC FOR IMPROVED MASS-TRANSFER OF LARGE MOLECULES; AND (2) TO TRANSFER THE RECENT TECHNOLOGY DEVELOPED BY DR. PIDGEON TO REGIS AND COMMERCIALIZE TWO NEW IAM PACKINGS; THE IAM.PC SURFACE END-CAPPED WITH ALKYLANHYDRIDES, WHICH STERICALLY PROTECT THE SILICA SURFACE FROM SOLVOLYSIS; AND AN IAM SURFACE CONTAINING A SINGLE ETHER-LINKED PHOSPHOLIPID. DR. PIDGEON'S EIGHT STEP ETHER PHOSPHOLIPID SYNTHESIS WILL BE SCALED UP FOR PRODUCTION AND COMMERCIALIZATION OF IAM SURFACES. THE LARGE SCALE LIPID SYNTHESIS WILL PROVIDE THE BASIS FOR THE SYNTHESIS OF ADDITIONAL IAM BONDED PHASES WITH DIFFERENT MEMBRANE LIPIDS DURING PHASE II. THIS IAM SURFACE DIVERSITY IS EXPECTED TO PROVIDE SELECTIVITY FOR MANY MEMBRANE PROTEINS, THUS EFFECTING THEIR PURIFICATION.

* information listed above is at the time of submission.

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