DEVELOPMENT OF RECOMBINANT FERTILITY VACCINE FOR FARM ANIMALS

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: N/A
Agency Tracking Number: 26890
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1994
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
3900 Collins Rd, Lansing, MI, 48909
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Dr Mira Bagdasarian
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
AT PRESENT THERE IS NO SAFE, CHEAP AND EFFECTIVE COMMERCIAL METHOD TO ENHANCE FERTILITY IN FARM ANIMALS. PREVIOUS WORK AT MICHIGAN STATE UNIVERSITY HAS SHOWN THAT IMMUNONEUTRALIZATION OF INHIBIN (A GONADAL GLYCOPROTEIN THAT SUPPRESSES SECRETION OF FOLLICLE STIMULATING HORMONE) ENHANCES: OVULATION RATE IN SHEEP, HEIFERS AND GILTS; FECUNDITY IN GILTS AND SPERMATOGENESIS IN BULLS. ALTHOUGH THIS VACCINE PROVED VALUABLE AS A FERTILITY-ENHANCING AGENT IT HAS NO COMMERICIAL MERIT SINCE IT CONSISTS OF CHEMICALLY SYNTHESIZED PEPTIDES CONJUGATED TO HUMAN A-GLOBULIN AND REQUIRES THE USE OF FREUND'S ADJUVANT. THIS PROJECT PROPOSES TO CONSTRUCT AND TEST RECOMBINANT VACCINES COMPOSED OF THE B SUBUNIT (ETXB) OF THE E. COLI HEAT-LIABLE ENTEROTOXIN AND ONE OR TWO IMMUNO-DOMINANT EPITOPES OF INHIBIN. THE CHIMERIC VACCINES WILL BE CONSTRUCTED BY INSERTION OF CHEMICALLY SYNTHESIZED OLIGONUCLEOTIDES (DESIGNED TO ENCODE APPROPRIATE EPITOPES) INTO THE GENE ENCODING ETXB PROTEIN SUCH THAT THE INSERTED PEPTIDES ARE EXPOSED AT THE SURFACE OF THE ETXB MOLECULE. CHIMERIC PROTEINS WILL BE PRODUCED BY BACTERIAL FERMENTATION, PURIFIED FROM THE MEDIUM AND TESTED FOR IMMUNOGENICITY IN GILTS.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government