Electrokinetic-based Microfluidic Universal Sample-Preparation (EMUS) Platform

Award Information
Agency:
Department of Defense
Branch
Defense Threat Reduction Agency
Amount:
$99,985.00
Award Year:
2010
Program:
SBIR
Phase:
Phase I
Contract:
HDTRA1-10-P-0008
Agency Tracking Number:
T092-002-0067
Solicitation Year:
2009
Solicitation Topic Code:
DTRA092-002
Solicitation Number:
2009.2
Small Business Information
CFD Research Corporation
215 Wynn Dr., 5th Floor, Huntsville, AL, 35805
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
Y
Duns:
185169620
Principal Investigator:
Ketan Bhatt
Research Engineer
(256) 327-0666
tsb@cfdrc.com
Business Contact:
Deborah Phipps
Senior Contracts Specialist
(256) 726-4800
dap@cfdrc.com
Research Institution:
n/a
Abstract
Sample preparation has been recognized as the single most important challenge to be faced in the development of detect-to-warn (DTW) systems. Available commercial sample preparation technologies are expensive, slow, and require trained laboratory technicians and sophisticated laboratory equipment for operation. Addressing this need, we propose to design, fabricate, and demonstrate a general purpose Electrokinetic-based Microfluidic Universal Sample-preparation platform (EMUS) for rapid, automated, simultaneous extraction of nucleic acids and proteins from complex environmental samples. The EMUS platform will be build on CFDRC’s expertise and proprietary technology for continuous sorting of biological particles, reagent-less electrokinetic lysing of cells and active mixing enhanced bead-based extraction of nucleic acids from cell samples. The EMUS platform will also incorporate modules for the removal of chemical contaminants from cell samples using dielectrophoretic filtration and for separation and enrichment of proteins using microdialysis. Proof-of-concept during Phase I will be established by separating target cells from biological and particulate clutter in a continuous fashion, by removal of PCR inhibitors from samples using DEP filtration and by desalting of protein samples using microdialysis. During Phase II, the fully integrated EMUS platform will be tested against a myriad of complex environmental samples.

* information listed above is at the time of submission.

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