Electrokinetic-based Microfluidic Universal Sample-Preparation (EMUS) Platform

Award Information
Agency: Department of Defense
Branch: Defense Threat Reduction Agency
Contract: HDTRA1-10-P-0008
Agency Tracking Number: T092-002-0067
Amount: $99,985.00
Phase: Phase I
Program: SBIR
Awards Year: 2010
Solicitation Year: 2009
Solicitation Topic Code: DTRA092-002
Solicitation Number: 2009.2
Small Business Information
CFD Research Corporation
215 Wynn Dr., 5th Floor, Huntsville, AL, 35805
DUNS: 185169620
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 Ketan Bhatt
 Research Engineer
 (256) 327-0666
 tsb@cfdrc.com
Business Contact
 Deborah Phipps
Title: Senior Contracts Specialist
Phone: (256) 726-4800
Email: dap@cfdrc.com
Research Institution
N/A
Abstract
Sample preparation has been recognized as the single most important challenge to be faced in the development of detect-to-warn (DTW) systems. Available commercial sample preparation technologies are expensive, slow, and require trained laboratory technicians and sophisticated laboratory equipment for operation. Addressing this need, we propose to design, fabricate, and demonstrate a general purpose Electrokinetic-based Microfluidic Universal Sample-preparation platform (EMUS) for rapid, automated, simultaneous extraction of nucleic acids and proteins from complex environmental samples. The EMUS platform will be build on CFDRC’s expertise and proprietary technology for continuous sorting of biological particles, reagent-less electrokinetic lysing of cells and active mixing enhanced bead-based extraction of nucleic acids from cell samples. The EMUS platform will also incorporate modules for the removal of chemical contaminants from cell samples using dielectrophoretic filtration and for separation and enrichment of proteins using microdialysis. Proof-of-concept during Phase I will be established by separating target cells from biological and particulate clutter in a continuous fashion, by removal of PCR inhibitors from samples using DEP filtration and by desalting of protein samples using microdialysis. During Phase II, the fully integrated EMUS platform will be tested against a myriad of complex environmental samples.

* information listed above is at the time of submission.

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