Development of Bioassays for Prion Infectivity Using Human, Deer, or Elk Cells

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$749,992.00
Award Year:
2005
Program:
STTR
Phase:
Phase II
Contract:
W81XWH-04-C-0138
Award Id:
68462
Agency Tracking Number:
A045-027-0018
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1008 32nd Ave, Brookings, SD, 57006
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
939726592
Principal Investigator:
Chris Mateo
Manager, Operations
(605) 692-6953
cmateo@ruraltechinc.com
Business Contact:
Christopher Chase
President
(605) 692-6953
rtilab@ruraltechinc.com
Research Institution:
NATIONAL ANIMAL DISEASE CENTER
Adrianna D Hewings
2300 Dayton Avenue
Ames, IA, 50010
(309) 681-6602
Domestic nonprofit research organization
Abstract
The human infectivity of Chronic Wasting Disease (CWD) is unclear. Current diagnostics rely upon post-mortem examination and late-term disease. Migratory B cells are emerging as likely candidates for blood-borne infectivity, and therefore, a logical target for sensitive antemortem diagnosis. We have focused on the Follicular Dendritic Cell (FDC)-B cell interaction as a target to expand prions. This approach has been verified using ovine cells infected with scrapie, and during Phase I we have cultured deer and elk FDCs to define an analagous assay for CWD. The goals of Phase II are to optimize this assay in cervid FDC cultures, and to quantify the sensitivity and specificity of prion replication. To accomplish these goals, we will 1) Confirm the applicability of the ovine assay to cervid tissues; 2) Optimize efficiency suitable to a commercial diagnostic test. 3) Define the limits of the system. 4) Define applicability to diagnosis of other tissues 5) Investigate the utility of the assay to evaluate the "species barriers" of CWD. At the conclusion of Phase II, we will deliver a streamlined in vitro culture assay for early blood-based diagnosis of prion disease in cervids, and applicability of the assay to other prion sources and species.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government