Protein Production in Tobacco from an Amplified Inducible System

Award Information
Agency:
Department of Defense
Amount:
$99,438.00
Program:
STTR
Contract:
W911NF-06-C-0105
Solitcitation Year:
2006
Solicitation Number:
N/A
Branch:
Army
Award Year:
2006
Phase:
Phase I
Agency Tracking Number:
A064-015-0073
Solicitation Topic Code:
A06-T015
Small Business Information
RXOA BIOSCIENCES LLC
1212 Fourier Drive, Madison, WI, 53717
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
162762913
Principal Investigator
 William Petersen
 (608) 229-2833
 williamljpt@aol.com
Business Contact
 William Petersen
Title: Managing Member
Phone: (608) 229-2833
Email: williamljpt@aol.com
Research Institution
 ARIZONA BOARD OF REGENTS, ASUNIV.
 Candyace Lindsay
 P.O. Box 873503
Tempe, AZ, 85287-3503
 (408) 727-0749
 Nonprofit college or university
Abstract
This Phase I proposal will test a UV-B light inducible system in tobacco to produce exceptionally large amounts of protein. There are three principal components to the system: 1) a tightly regulated, UV-B inducible promoter fused to a geminivirus replication associated protein (Rep) coding region, 2) a geminivirus-derived vector that is rapidly amplified by the Rep protein to produce very high yields of the target protein, and 3) plant growth under light-emitting diode (LED) lighting for precise control of the expression system. After sufficient plant biomass accumulation, exposure to UV-B will trigger production of Rep. The Gemini vector DNA will then be precisely excised from the plant genome and replicated episomally, leading to very high intracellular concentrations of transgene mRNA and the encoded transgenic protein product. The specific aims of the Phase I proposal are to: 1 Assemble UV-B inducible genetic constructs to express Rep protein and introduce them into tobacco plants. 2 Test a prototype LED-based lighting system for plant biomass production. 3 Test background expression levels and upon UV-B activation, test the ability of the constructs to induce the geminivirus based expression system in tobacco. 4 Determine the total amount of protein produced per gram of plant biomass.

* information listed above is at the time of submission.

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