Development of Novel Flow Cytometer for Biological Warfare Agent Detection

Award Information
Agency: Department of Defense
Branch: Defense Advanced Research Projects Agency
Contract: N/A
Agency Tracking Number: 31760
Amount: $98,374.00
Phase: Phase I
Program: SBIR
Awards Year: 1996
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Science & Engineering
4014 Blackburn Lane, Burtonsville, MD, 20866
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Hyo Sang Lee
 (301) 989-1896
Business Contact
Phone: () -
Research Institution
N/A
Abstract
SESI proposes the development of a novel flow cytometer to detect biological warfare agents in large multiplex of up to 300 agents. Our proposed cytometer combines new phosphorescence technology in conjunction with a state-of-the-art flow cytometer configuration to achieve simultaneous 300-agent multiplexing without further complication of the cytometer design. Up to 60 agents could be simultaneously detected in a flow cytometer equipped with only an upconversion phosphor channel and forward scattering. An innovative feature of our channel as a baseline operation, as well as phosphor channel performance using the fundamental laser wavelength. In addition, the flow cytometer configuration will implement an electric cell volume measurement capability for accurate sizing of individual cells. The proposed cytometer optically, and electrically, probes individual cells at rates of up to 1000 cells per second. This flow speed of the cytometer allows continuous point sampling and monitoring. Second generation advances in miniaturization, (which will speed up assay kinetics and reduce the sample volumes required to achieve detection) may permit use of this system on mobile platforms, such as the UAV.

* information listed above is at the time of submission.

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