Automated Sample Collection and Concentration System for Multiple Pathogens in Water

Award Information
Agency:
Environmental Protection Agency
Branch
n/a
Amount:
$344,938.00
Award Year:
2007
Program:
SBIR
Phase:
Phase II
Contract:
EP-D-07-089
Award Id:
79844
Agency Tracking Number:
EP-D-06-055
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
12441 Beckley St, Granger, IN, 46530
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
148374627
Principal Investigator:
Fu-ChihHsu
Director of Operations
(574) 277-4078
fuchih@scientificmethods.com
Business Contact:
RubyLarkin
Chief Financial Officer
(574) 277-4078
ruby@scientificmethods.com
Research Institute:
n/a
Abstract
The goal of this research project is to develop a simple, rapid and highly automated sample collection system that will concentrate a broad range of pathogens simultaneously. The system will integrate continuous flow centrifugation (CFC) with an innovative positively charged filter so that large (i.e., protozoan parasites and bacteria) and small (i.e., enteric viruses) bioparticles can be concentrated under an integrated platform. Because CFC is not subject to the limitations of traditional filtration approaches that are subject o fouling, and because its utility was evaluated extensively through Phase I study using a broad range of waterborne pathogens, the integration of CFC and cartridge filtration will extend the utility of the sample concentration platform to accommodate a diverse range of natural and treated water samples that cannot be processed using current filtration methods alone. The system developed in this research project is intended to be compatible with current and commonly employed analytic detection techniques and other advanced detection methods such as real-time PCR, DNA microchip arrays, as well as other biosensors. This innovative sample collection and concentration system will provide concentrated samples for routine and advanced water quality monitoring, and will be readily incorporated as a front-end measure for rapid identification of bio-agents in the face of bioterrorism events and post attack monitoring. The final product is envisioned as an automated sample collection and concentration device that will accommodate a variety of water matrices ranging form finished drinking waters to particle-rich matrices such as surface and waste waters. The open design of the automated system will also extend the utility of on-line and real-time sensor arrays for waterborne pathogens and indicator microorganisms.

* information listed above is at the time of submission.

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