BIOLUMINESCENT ASSAY FOR GENE EXPRESSION ANALYSIS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 2R44GM080037-02
Agency Tracking Number: GM080037
Amount: $728,798.00
Phase: Phase II
Program: SBIR
Awards Year: 2008
Solicitation Year: 2008
Solicitation Topic Code: N/A
Solicitation Number: PHS2007-2
Small Business Information
SCI-TEC, INC.
10425 COGDILL RD., #300, KNOXVILLE, TN, 37932
DUNS: 015180610
HUBZone Owned: Y
Woman Owned: Y
Socially and Economically Disadvantaged: Y
Principal Investigator
 () -
Business Contact
Phone: (865) 671-2166
Email: golovlev@scien-tec.com
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Bioluminescent Assay (RT-qBLA) for high-throughput detection and quantification of RNA and DNA in biological samples. The assay imple ments the same detection concept known from pyrosequencing, yet expanding pyrosequencing methodology for highly sensitive and accurate quantification of RNA and DNA molecules. The proposed assay has unique sensitivity and dynamic range and is able to measu re kinetics of RNA and DNA replication in the solution in real time without long and expensive sample amplification of the real time PCR. The RT-qBLA requires less expensive reagents and equipment than real time PCR and DNA microarrays and can be used in a various commercial assays in life sciences research, drug discovery, and clinical diagnosis.

* information listed above is at the time of submission.

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