IN VITRO SCREENING TEST FOR DETECTING ANTIESTROGENS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
7168
Agency Tracking Number:
7168
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
12111 Parklawn Drive, Rockville, MD, 20852
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ARULASANAM K THILAGAR PHD
(301) 984-2302
Business Contact:
() -
Research Institute:
n/a
Abstract
THE OVERALL OBJECTIVE OF THIS PROPOSAL IS TO DEVELOP A RELIABLE IN VITRO TEST SYSTEM USING HUMAN CELL LINES TO SCREEN COMPOUNDS FOR THEIR CYTOTOXICITY AND ANTIESTROGENIC ACTIVITY. CURRENTLY, A NUMBER OF IN VITRO SCREENING TESTS ARE USED TO IDENTIFY POTENTIAL CHEMOTHERAPEUTIC DRUGS. MOST OF THESE TESTS UTILIZE NONHUMAN (MOSTLY RODENT) CELL LINES THAT MAY NOT DISTINGUISH SPECIES-SPECIFIC DIFFERENCES IN TOXICITY OF CHEMICALS BETWEEN HUMANS AND RODENTS. ALTHOUGH A FEW HUMAN CELL LINES (HT-29, A549, KB) ARE CURRENTLY USED TO SCREEN CHEMICALS FOR THEIR CYTOTOXICITIES, NONE OF THEM ARE RESPONSIVE TO ESTROGENS OR ANTIESTROGENS. BECAUSE IN RECENTYEARS PATIENTS WITH HORMONE-RESPONSIVE SOLID TUMORS HAVE BEEN TREATED WITH BOTH ENDOCRINE DRUGS (ANTIESTROGENS) AND OTHER CHEMOTHERAPEUTIC AGENTS, IT IS ESSENTIAL TO DEVELOP ANIN VITRO SCREENING TEST WITH APPROPRIATE HUMAN ESTROGEN-SENSITIVE AND ESTROGEN-NONSENSITIVE CELL LINES. USE OF HUMAN CELL LINES WILL ALSO BE MORE RELEVANT IN VIEW OF THE DIFFERENCES IN THE SPECIES-SPECIFIC CYTOTOXICITY OF ANUMBER OF COMPOUNDS. SITEK RESEARCH LABORATORIES PROPOSES TO DEVELOP A RELIABLE PROTOCOL FOR A RAPID, IN VITRO TEST USING ESTROGEN-RESPONSIVE AND ESTROGEN-NONRESPONSIVE HUMAN CELL LINES. SUCH A TEST WILL BE SENSITIVE, RELATIVELY INEXPENSIVE, AND ALLOW THE EVALUATION OF A NUMBER OF SAMPLESAT THE SAME TIME. IN ADDITION, THE TEST WILL DISTINGUISH THE TOXICITY CAUSED BY THE ANTIESTROGENS FROM THE NONSPECIFIC CYTOTOXICITY OR TOXICITY CAUSED BY OTHER CHEMOTHERAPEUTIC DRUGS.

* information listed above is at the time of submission.

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