IN VITRO SCREENING TEST FOR DETECTING ANTIESTROGENS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7168
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1987
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Sitek Research Laboratories
12111 Parklawn Drive, Rockville, MD, 20852
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 ARULASANAM K THILAGAR PHD
 (301) 984-2302
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE OVERALL OBJECTIVE OF THIS PROPOSAL IS TO DEVELOP A RELIABLE IN VITRO TEST SYSTEM USING HUMAN CELL LINES TO SCREEN COMPOUNDS FOR THEIR CYTOTOXICITY AND ANTIESTROGENIC ACTIVITY. CURRENTLY, A NUMBER OF IN VITRO SCREENING TESTS ARE USED TO IDENTIFY POTENTIAL CHEMOTHERAPEUTIC DRUGS. MOST OF THESE TESTS UTILIZE NONHUMAN (MOSTLY RODENT) CELL LINES THAT MAY NOT DISTINGUISH SPECIES-SPECIFIC DIFFERENCES IN TOXICITY OF CHEMICALS BETWEEN HUMANS AND RODENTS. ALTHOUGH A FEW HUMAN CELL LINES (HT-29, A549, KB) ARE CURRENTLY USED TO SCREEN CHEMICALS FOR THEIR CYTOTOXICITIES, NONE OF THEM ARE RESPONSIVE TO ESTROGENS OR ANTIESTROGENS. BECAUSE IN RECENTYEARS PATIENTS WITH HORMONE-RESPONSIVE SOLID TUMORS HAVE BEEN TREATED WITH BOTH ENDOCRINE DRUGS (ANTIESTROGENS) AND OTHER CHEMOTHERAPEUTIC AGENTS, IT IS ESSENTIAL TO DEVELOP ANIN VITRO SCREENING TEST WITH APPROPRIATE HUMAN ESTROGEN-SENSITIVE AND ESTROGEN-NONSENSITIVE CELL LINES. USE OF HUMAN CELL LINES WILL ALSO BE MORE RELEVANT IN VIEW OF THE DIFFERENCES IN THE SPECIES-SPECIFIC CYTOTOXICITY OF ANUMBER OF COMPOUNDS. SITEK RESEARCH LABORATORIES PROPOSES TO DEVELOP A RELIABLE PROTOCOL FOR A RAPID, IN VITRO TEST USING ESTROGEN-RESPONSIVE AND ESTROGEN-NONRESPONSIVE HUMAN CELL LINES. SUCH A TEST WILL BE SENSITIVE, RELATIVELY INEXPENSIVE, AND ALLOW THE EVALUATION OF A NUMBER OF SAMPLESAT THE SAME TIME. IN ADDITION, THE TEST WILL DISTINGUISH THE TOXICITY CAUSED BY THE ANTIESTROGENS FROM THE NONSPECIFIC CYTOTOXICITY OR TOXICITY CAUSED BY OTHER CHEMOTHERAPEUTIC DRUGS.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government