A RAPID IMMUNOASSAY FOR YERSINIA ENTERCOLITICA

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$30,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
7885
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Skyland Scientific Svcs Inc
2311 S 7th Ave, Bozeman, MT, 59715
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
FIVE MAJOR FOODBORNE OUTBREAKS OF YERSINIOSIS HAVE OCCURRED IN THE UNITED STATES WITHIN THE LAST DECADE AND THE DISEASE IS ENDEMIC IN MANY OTHER COUNTRIES OF THE WORLD. YERSINIA ENTEROCOLITICA IS OF PARTICULAR CONCERN TO THE FOODINDUSTRY BECAUSE IT IS A PSYCHROTROPH, AND, THEREFORE, GROWTH CAN NOT BE CONTROLLED BY REFRIGERATION. LABORATORY METHODOLOGY PRESENTLY AVAILABLE FOR DETECTING Y. ENTEROCOLITICA IS TIME-CONSUMING. TWO UNIQUE ANTIGENIC STRUCTURES ON PATHOGENIC STRAINS OF Y. ENTEROCOLITICA PRESENT THE BASIS FOR DEVELOPING AN IMMUNOASSAY FOR RAPID DETECTION OF Y. ENTEROCOLITICA: (I) UNIQUE OUTER MEMBRANE PROTEINS SYNTHESIZED AT 37 C BY STRAINS THAT CARRY A VIRULENCE PLASMID, AND (II) FLAGELLA. SPECIFIC ANTIBODY WILL BE INCORPORATED INTO TWO EXPERIMENTAL IMMUNOASSAYS: (I)A DISPERSE DYE SYSTEM, AND (II) AN AVIDIN-BIOTIN ENZYME SYSTEM. AN IMMUNOASSAY CAN BE ASSEMBLED AS A MARKETABLE TEST KIT FOR RAPID DETECTION OF Y. ENTEROCOLITICA IN FOODS. A LONG-TERM OBJECTIVE IS THE DEVELOPMENT OF ANTIGEN AND ANTIBODY DETECTION SYSTEMS FOR RAPID IDENTIFICATION OF Y. ENTEROCOLITICA AND MEASURING SEROLOGICAL RESPONSES IN CLINICAL MICROBIOLOGY.

* information listed above is at the time of submission.

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