SURFACE ENHANCED MICROCARRIERS FOR LOW-ADHESION CELLS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$575,344.00
Award Year:
1995
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
19133
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Solohill Engineering Inc.
1919 Green Rd, Ann Arbor, MI, 48105
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
William J Hillegas
(313) 665-0453
Business Contact:
() -
Research Institution:
n/a
Abstract
THE NEED FOR VERSATILE AND ECONOMICAL INDUSTRIAL SCALE TECHNOLOGIES FOR CULTURING ANCHORAGE-DEPENDENT MAMMALIAN CELLS IS BECOMING MORE EVIDENT. THIS IS A RESULT OF THE INCREASING DEMAND FOR VACCINES AND THE USE OF MAMMALIAN CELLS FOR SOME OF THE NEWLY EMERGING BIOPHARMACEUTIALS. MICROCARRIER BEADS IN BIOREACTOR SYSTEMS SHOW GREAT POTENTIAL FOR USE AS A LARGE SCALE PRODUCTION METHOD. SOLOHILL IS DEVELOPING A FAMILY OF UNIQUE MICROCARRIER PRODUCTS WHICH ARE SUPERIOR TO THOSE CURRENTLY AVAILABLE. THE AIM OF THIS RESEARCH IS TO DEVELOP A NOVEL MICROCARRIER BEAD WHICH WILL BE USEFUL WITH CERTIAN TYPES OF LOW-ADHESIVE, TRANSFORMED CELLS WHICH DO NOT READILY ATTACH AND SPREAD ON MICROCARRIERS. SUCH CELLS ARE FREQUENTLY USED IN THE MANUFACTURE OF VETERINARY AND HUMAN BIOLOGICALS. WE WILL MODIFY THE SURFACE OF OUR GELATIN-COATED MICROCARRIERS TO MAKE THEM MORE ADHESIVE TO LOW ADHESIVE TRANSFORMED CELLS WHILE MAINTAINING THE OTHER GROWTH CHARACTERISTICS THAT HAVE MADE GELATIN COATED MICROCARRIERS SUCCESSFUL. SPECIFICALLY THESE STUDIES WILL EVALUATE THREE NEW MICROCARRIERS SYNTHESIZED TO CONTAIN A COMBINATION OF GELATIN AND EITHER (1 A CATIONIC POLYAMINO ACID, (2) A STABLE SYNTHETIC PEPTIDE OR (3) HYDROXYAPATITE. IF SUCCESSFUL THE PRODUCT WILL EXTEDN THE BENEFITS OF MICROCARRIER TECHNOLOGY TO A WIDER MARKET.

* information listed above is at the time of submission.

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