Small Microcarrier Clumping Yields Higher Cell Densities

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$546,357.00
Award Year:
1996
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
22078
Agency Tracking Number:
22078
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1919 Green Road, Ann Arbor, MI, 48105
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
William J. Hillegas
(313) 665-0453
Business Contact:
() -
Research Institute:
n/a
Abstract
The need for versatile and economic industrial scale technologies for culturing anchorage-dependent mammalian cells is becoming more evident. This is a result of the increasing demand for vaccines and the use of mammalian cells for some of the new, emerging biopharmaceuticals. Microcarrier beads in bioreactor systems are being applied as a large scale production method. SoloHill is developing a family of unique microcarrier products which are superior to those currently available. The overall aim is to develop a novel small microcarrier bead (38-63 micrometers) which will foster the aggregation of cells. This will encourage the use of microcarriers for conditions where higher cell densities have been shown to be advantageous. Specifically we will (l) demonstrate that higher cell densities can be achieved under conditions where extensive clumping occurs. We will characterize the bead-to-bead clumps on small (less than 63 micrometers) and large (150-212 micrometers) beads. And, we will (2) demonstrate that cells maintained under conditions of high cell density on clumped beads are metabolically active and produce higher levels of useful products including infectious virus than cells grown under conventional conditions (in which clumping is minimized).

* information listed above is at the time of submission.

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