Microcarriers designed for protein & sera free media

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$98,116.00
Award Year:
2004
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM070195-01
Award Id:
71644
Agency Tracking Number:
GM070195
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
SOLOHILL ENGINEERING, INC., 4220 VARSITY DR, ANN ARBOR, MI, 48108
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
WILLIAM HILLEGAS
(734) 973-2956
SOLOHILL@IC.NET
Business Contact:
DAVID SOLOMON
(734) 973-2956
SOLOHILL@IC.NET
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): The overall goal of this study is to develop a microcarrier coated with a synthetic, 15 amino acid peptide sequence found in type I collagen. This microcarrier would replace current microcarriers utilizing native collagen or gelatin in protocols requiring animal product-free conditions. Currently three gelatin-coated microcarriers, a gelatin-linked dextran (Cytodex III, made by the United Kingdoms's Amersham-Pharmacia Biotechnology) and Solohill Engineering's two gelatin-coated polystyrene microcarriers, are the most extensively used microcarrier substrates in the industrial scale production of anchorage-dependent cells today. As manufacturers of viral vaccines and other biologicals move toward completely animal product-free manufacturing conditions, a substrate containing collagen or gelatin will not be acceptable. The synthetic-collagen-peptide coated microcarriers would provide an ideal replacement. In the Phase I portion of this SBIR grant application, we propose the following experimental program to demonstrate the feasibility of producing such a product. Specific Aim I. To attach a synthetic-collagen-peptide to the surface of polystyrene microcarrier core beads and to compare the synthetic peptide - coated microcarriers with currently available collagen-coated polystyrene microcarriers for ability to support attachment and growth of anchorage-dependent animal cells under serum-free conditions. Specific Aim II. To attach a synthetic-collagen-peptide to the surface of charged-surface polystyrene microcarrier core beads (Hillex microcarriers) and to compare the synthetic peptide-coated microcarriers with currently available charged-surface polystyrene microcarriers for ability to support attachment and growth of anchorage-dependent animals cells under serum-free conditions. Specific Aim III. To attach a synthetic-collagen-peptide to the surface of dextran microcarrier core beads and to compare the synthetic peptide-coated microcarriers with currently-available collagen-coated dextran microcarriers for ability to support attachment and growth of anchorage-dependent animals cells under serum-free conditions.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government