A TWO-SITE IMMUNOASSAY WILL BE DEVELOPED AGAINST APOLIPO- PROTEIN A-I (APOA-I), THE MAJOR APOPROTEIN OF HIGH-DENSITY LIPOPROTEIN (HDL).

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
5000
Agency Tracking Number:
5000
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
12815 Ne 124th Street, Kirkland, WA, 98304
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
PATRIC A CLAPSHAW
PRINCIPAL INVESTIGATOR
(206) 821-7005
Business Contact:
() -
Research Institute:
n/a
Abstract
A TWO-SITE IMMUNOASSAY WILL BE DEVELOPED AGAINST APOLIPO- PROTEIN A-I (APOA-I), THE MAJOR APOPROTEIN OF HIGH-DENSITY LIPOPROTEIN (HDL). LOW LEVELS OF APOA-I HAVE BEEN POSITIVELY CORRELATED WITH CORONARY ARTERY DISEASE (CAD). TO ENSURE ADEQUATE QUANTIFICATION, CYANOGEN BROMIDE (CNBR) FRAGMENTS OF APOA-I WILL BE USED TO GENERATE MONOCLONAL ANTIBODIES (MABS), WHICH WILL BE SELECTED TO RECOGNIZE SEPARATE EPITOPES ON THE SOLUBLE PROTEIN IN UNTREATED PLASMA. THE ASSAY SYSTEM USED WILL BE THE ENZYME-LINKED IMMUNOSORBANT ASSAY (ELISA). QUANTITATIVE RESULTS FROM THE ELISA WILL BE CORRELATED WITH OTHER IMMUNOASSAYS AND WITH ASSAYS BASED ON DIRECT MEASUREMENT OF PROTEIN MASS. THE ULTIMATE GOAL WILL BE TO DEVELOP ELISA KITS FOR RESEARCH ANDCLINICAL MEASUREMENT OF THIS IMPORTANT CAD RISK FACTOR.

* information listed above is at the time of submission.

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