BIOSYNTHESIS OF A NEW ANTICHOLESTEROLGENIC COMPOUND

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
8072
Agency Tracking Number:
8072
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
350 Interlocken Pky #100, Broomfield, CO, 80020
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institute:
n/a
Abstract
THE LONG TERM OBJECTIVE OF THIS PROPOSAL IS TO DEVISE A METHOD FOR THE LARGE SCALE BIOSYNTHESIS OF A NEGATIVE REGULATOR OF MAMMALIAN CHOLESTEROL BIOSYNTHESIS. THIS COMPOUND HAS POTENTIAL USE IN THE TREATMENT OF HYPERCHOLESTEROLEMIA AND MAY REDUCE THE OCCURRENCE OF ATHEROSCLEROTIC HEART DISEASE. AS A STEP TOWARD ACHIEVING THE BIOSYNTHESIS OF THIS COMPOUND, WE PROPOSE TO ISOLATE THEGENE ENCODING A KEY ENZYME REQUIRED FOR THE SYNTHESIS OF THIS REGULATORY MOLECULE, DETERMINE THE COMPLETE NUCLEOTIDE SEQUENCE OF THE GENE AND THE LOCATION OF TRANSCRIPTIONAL INITIATION. THE GENE WILL BE CLONED FROM THE YEAST SACCHAROMYCES CEREVISIAE BY COMPLEMENTATION OF AN AUXOTROPHIC MUTATION IN THAT GENE OR BY OVERCOMING THE EFFECTS OF A SPECIFIC ENZYMATIC INHIBITOR THROUGH GENE DOSAGE. THE CLONE, AND THE INFORMATION PROVIDED BY ITS SEQUENCE, WILL BE USED TO CONSTRUCT A MICROBIAL STRAIN CAPABLE OF PRODUCING THE REGULATORY MOLECULE. SUCH A STRAINMAY ALLOW COMMERCIAL SCALE PRODUCTION OF THE COMPOUND FOR FURTHER TESTING AND DEVELOPMENT.

* information listed above is at the time of submission.

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