Cassette Based Robotic Multiple Cell Culture System

Award Information
Agency:
National Aeronautics and Space Administration
Branch
n/a
Amount:
$599,982.00
Award Year:
2002
Program:
SBIR
Phase:
Phase II
Contract:
NAS9-02081
Agency Tracking Number:
012278
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Space Hardware Optimization Technology
7200 Highway 150, Greenville, IN, 47124
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr Paul Todd
Chief Scientist
(812) 923-9591
ptodd@shot.com
Business Contact:
Mark Deuser
President & CEO
(812) 923-9591
mdeuser@shot.com
Research Institution:
n/a
Abstract
The specific innovation proposed for continued research is a family of fully-automated (robotic) cell-culture systems, each a laboratory in a cassette, named collectively ?Multicult?. SHOT will incorporate several technical innovations, including options for gentle mixing of cell suspensions, transparent monolayer culture surfaces, control of inertial load (?g-level?) by rotation within the cassette, pH monitoring, adding fresh medium or fixatives with immediate mixing, and real-time imaging of cells in transparent containers. SHOT will perform research leading to (1) a cassette-based rotating plate for exposing 8 cultures of suspended cells to acceleration levels from 1 to 5 g while facilitating fluid additions and balancing out all forces other than centrifugal and gravity vectors, (2) a curved-wall transparent culture vessel for centrifugal studies of adherent cells, (3) a miniaturized non-invasive pH reader, (4) a compact video microscope, (5) a control-and-data network of embedded processors, and (6) a deliverable prototype cassette-based Multicult system incorporating these features for the sponsor. SHOT will use the results of this research to develop, in addition to a cell culture capability for space flight, marketable instruments based on its pH reader, centrifugal culture plates and autonomously controlled Multicults.

* information listed above is at the time of submission.

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