Microfluidic Analytical Separator for Proteomics

Award Information
Agency:
National Aeronautics and Space Administration
Branch
n/a
Amount:
$69,992.00
Award Year:
2004
Program:
SBIR
Phase:
Phase I
Contract:
NNJ04JC09C
Award Id:
68496
Agency Tracking Number:
033429
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
7200 Highway 150, Greenville, IN, 47124
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
621970383
Principal Investigator:
MarkDeuser
Principal Investigator
(812) 923-9591
mdeuser@SHOT.com
Business Contact:
MarkDeuser
President/CEO
(812) 923-9591
mdeuser@SHOT.com
Research Institute:
n/a
Abstract
SHOT proposes an innovative microfluidic device designed to effect a 2-dimensional resolution of a mixture of proteins based on isoelectric point (pI) and molecular weight. A novel approach is proposed to achieve 2-D-gel equivalence. The first dimension is to be immobilized isoelectric focusing, and the second dimension will utilize an array of 100+ size-exclusion chromatography columns in microchannels. Molecular weight is recorded as each protein leaves each microcolumn. The output column number can be translated into pI. Thus each combination of output peak position and column number gives the combined values of pI and MW for each protein in the same way as does a ?spot? on a 2-D electrophoresis gel. Phase I research objectives are to (1) demonstrate the measurement of protein concentration in microchannels using a proposed electrical impedance method (2) construct and test immobiline channels and demonstrate their function in determining pI (3) construct a size-exclusion chromatography channel and demonstrate its function in determining molecular weight, and (4) identify fabrication steps for the integrated ?chip? to be tested in Phase II research. Also in Phase II research the device will be incorporated into an automatically controlled cassette configuration for operation in space flight and in the laboratory.

* information listed above is at the time of submission.

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