CYCLIC AMP BINDING PROTEINS AND RAS FUNCTION

Award Information
Agency:
Department of Health and Human Services
Branch:
N/A
Amount:
$50,000.00
Award Year:
1992
Program:
SBIR
Phase:
Phase I
Contract:
N/A
Agency Tracking Number:
19449
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Sphinx Pharmaceuticals Corp
Two University Place, Po Box 5233, Durham, NC, 27717
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
N/A
Principal Investigator
 Men-hwei Tsai
 (919) 489-0909
Business Contact
Phone: () -
Research Institution
N/A
Abstract
TWO CAMP BINDING PROTEINS (50K AND 10K DALTONS) THAT ALTER BIOCHEMICAL PROPERTIES OF PROTOON- COGENE RAS IN A CAMP DEPENDENT MANNER WERE ISOLATED FROM BOVINE BRAIN. IN THE PRESENCE OF CAMP, THE 50K DALTON PROTEIN (P50) INHIBITED THE NUCLEOTIDE EXCHANGE OF RAS, WHEREAS THE 10K PROTEIN (P10) INHIBITED NUCLEOTIDE BINDING. IN THE ABSENCE OF CAMP, THESE TWO PROTEINS ARE TOTALLY INACTIVE. IT IS WELL ESTABLISHED THAT PROTOONCOGENE RAS PLAYS AN IMPORTANT ROLE IN CONTROLLING CELL PROLIFERATION AND DIFFERENTIATION. THE INTERACTION BETWEEN THESE TWO PROTEINSAND RAS MAY REPRESENT A NOVEL MECHANISM BY WHICH CELL PROLIFERATION AND DIFFERENTIATION. THE INTERACTION BETWEEN THESE TWO PROTEINS AND RAS MAY REPRESENT A NOVEL MECHANISM BY WHICH CELL PROLIFERATION AND DIFFERENTIATION ARE REGULATED. THE OBJECTIVE OF THIS PHASE I PROJECT IS TO PARTIALLY PURIFY THESE TWO PROTEINS, ESTABLISHED A STANDARD ASSAY AND SCREEN FOR THE COMPOUND OR NATURAL PRODUCTS THAT ARE ABLE TO ALTER THE BIOCHEMICAL AND BIOLOGICAL ACTIVITY OF THESE TWO PROTEINS. THE COMPOUNDS IDENTIFIED IN THIS PHASE I STUDY MAY DEVELOP INTO POTENTIAL ANTI- PROLIFERATIVE DRUGS. IN THE PHASE II PORTION OF THIS GRANT, THESE TWO PROTEINS WILL BE PURIFIED, SEQUENCED AND CLONED. THE BIOLOGICAL SIGNIFICANCE OF THESE TWO PROTEINS WILL BE ELUCIDATED BY INTRODUCTING THESE TWO PROTEINS INTO CULTURED CELLS BY PROTEIN MICROINJECTION AND/OR GENE TRANSFER TECHNIQUES. ALTERNATIVELY, ANTIBODIES THAT ARE ABLE TO NEUTRALIZE THESE TWO PROTEINS WILL BE MICROINJECTED INTO CULTURED CELLS TO DETERMINE THE BIOLOGICAL FUNCTION OF THESE TWO PROTEINS. THE CELL MODEL WILL BE ESTABLISHED TO SCREEN THE COMPOUNDS THAT ARE ABLE TO ALTER THE BIOLOGICAL ACTIVITY OF THESE TWO PROTEINS.

* information listed above is at the time of submission.

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