MEMBRANE-BOUND RAD P21 REGULATION BY GAP AND LIPIDS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1992
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
19072
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Sphinx Pharmaceuticals
Po Box 52330, Durham, NC, 27717
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Stephen B Bocckino
(919) 489-0909
Business Contact:
() -
Research Institution:
n/a
Abstract
THE LONG-TERM GOAL OF THIS PROJECT IS TO DISCOVER DRUGS THAT WILL PROTECT NORMAL CELLS FROM CANCER CHEMOTHERAPY. THE ONCOGENE RAS IS PRESENT IN APPROXIMATELY 30% OF HUMAN TUMORS; A NORMAL NON-ONCOGENIC RAS IS FOUND IN NORMAL CELLS. THIS NORMAL RAS (BUT NOT ONCOGENIC RAS) CAN BE SWITCHED OFF BY THE CYTOSOLIC PROTEIN GAP RESULTING IN INHIBITION OF CELL DIVISION. THIS DIFFERENCE CAN BE EXPLOITED BY COMPOUNDS THAT ACT THROUGH GAP-RAS TO INHIBIT THE PROLIFERATION OF NORMAL CELLS, SPARING THEM FROM THE EFFECTS OF ANTI-PROLIFERATIVE THERAPY. MOST PREVIOUS WORK ON GAP-RES INTERACTIONS HAS UTILIZED SOLUBLE RAS. SINCE LIPIDS APPEAR TO REGULATE THE GAP-RAS INTERACTION, IT IS IMPORTANT TO USE THE MORE PHYSIOLOGICAL MEMBRANE-BOUND FORM OF RAS P21. IN PHASE I OF THIS SBIR GRANT, AN ASSAY WILL BE CONSTRUCTED FOR MEMBRANE-BOUND NON-ONCOGENIC RAS PROTEIN AND ITS REGULATION BY GAP (SPECIFIC AIM 1). THE MEMBRANE-BOUND RAS WILL THEN GE USED TO STUDY THE EFFECTS OF REGULATORY LIPIDS (SPECIFIC AIM 2). SUBSEQUENT TO PHASE I, COMPOUNDS ACTING TO INHIBIT NORMAL RAS FUNCTION WILL BE IDENTIFIED, AND TESTED IN RELEVANT CELL AND ANIMAL MODELS.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government