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Automatic Quantification of High S:N Images Using VCS

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43EB001605-01
Agency Tracking Number: MH076541
Amount: $98,840.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2003
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (410) 490-2036
Business Contact
Phone: (410) 490-2036
Research Institution

DESCRIPTION (provided by applicant):
Theoretically unbiased stereology has been applied to the morphometric analysis of biological tissue sections in the Stereologer, a commercially available, integrated hardware-software-microscopy system. A general limitation of this and other such systems lies in the time- and labor-intensive requirement for identification of targeted objects by trained non-expert users. This Phase 1 SBIR will test the accuracy, efficiency, and feasibility of a novel modification -- Verified Computerized Stereoanalysis (VCS) - for automatic quantification of stereological parameters for objects with a high signal-to-noise ratio (S:N). This automatic combination of image analysis and unbiased stereology will be implemented on the Stereologer system and evaluated through a two-step process: systematic-random sampling under automatic stage control; and, automatic quantification of mean object size and variation. To avoid the collection of spurious data, a verification process after step one will ensure that minimal S:N of equal to or > 90% exists prior to automatic data collection. Accuracy will be evaluated through parallel automatic and semi-automatic analyses on routine tissue sections and by external beta test sites. The VCS approach has the potential to drastically improve throughput of stereological studies, without a loss of accuracy, and thereby stimulate progress in a wide range of biomedical research projects.

* Information listed above is at the time of submission. *

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